Anti-RAD51B Polyclonal Antibody for WB and ELISA - O15315

Core Product Specifications and Parameters

Biological Background: RAD51B Function and Localization

• RAD51B (DNA repair protein RAD51 homolog 2) is a member of the RAD51 paralog family, sharing structural and functional similarities with the central recombinase RAD51. It is also known as RAD51L1, REC2, and R51H2.
• It plays a critical role in the homologous recombination repair (HRR) pathway, facilitating the repair of double-stranded DNA breaks that occur during DNA replication or are induced by genotoxic agents.
• RAD51B is a key component of the BCDX2 complex (RAD51B-RAD51C-RAD51D-XRCC2), which functions downstream of BRCA2 recruitment and upstream of RAD51 filament formation. The complex binds to Holliday junctions and replication fork junctions, aiding in the resolution of recombination intermediates.
• The RAD51B:RAD51C subcomplex exhibits single-stranded DNA-dependent ATPase activity, suggesting its involvement in the early presynaptic stage of HR.
• RAD51B binds both single-stranded and double-stranded DNA and possesses DNA-dependent ATPase activity, indicative of its role in nucleoprotein filament dynamics.
• Localized primarily in the nucleus, RAD51B is expressed across a wide range of tissues, underscoring its fundamental role in genome maintenance.
• Post-translational modifications include phosphorylation, and the protein features conserved ATP-binding and DNA-binding domains essential for its repair function.
• Keywords associated with RAD51B include DNA damage, DNA repair, DNA recombination, nucleotide-binding, and phosphoprotein, highlighting its central role in the DNA damage response.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of RAD51B is approximately 42 kDa. Use appropriate protein loading controls and validate band specificity in your lysate system, as the antibody is polyclonal and may detect additional bands.
• Given its reactivity with mouse and rat samples, this antibody can be used for cross-species studies in DNA repair research. However, always confirm cross-reactivity in your specific experimental conditions.
• In ELISA applications, consider optimizing coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratios.
• As the immunogen corresponds to full-length human RAD51B (aa 1-384), the antibody is expected to recognize multiple epitopes, potentially increasing sensitivity but also requiring careful blocking and washing steps to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-materials partner, connecting you to validated antibodies like this anti-RAD51B pAb for DNA damage and cancer research. Beyond individual antibodies, we streamline procurement of antibody pairs, bulk ancillary reagents, and even rare-target sourcing to accelerate your assay development—from concept to commercialization. Let our supply network support your next genotoxicity or oncology project with reliable, scalable raw materials.