Anti-RAD51 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q06609

Core Product Specifications and Parameters

Biological Background: RAD51 Function and Localization

• RAD51 is the human homolog of the bacterial RecA protein and a key mediator of homologous recombination.
• It binds single-stranded DNA in an ATP-dependent manner, forming nucleoprotein filaments that perform homology search and strand exchange during DNA double-strand break repair. Related references: PMID:12205100, PMID:15937124, PMID:17515904
• RAD51 displaces replication protein-A (RPA) from ssDNA and stabilizes RAD51-ssDNA filaments by blocking ATP hydrolysis. Related references: PMID:20729859
• It catalyzes joint molecule formation between processed DNA ends and homologous repair templates. Related references: PMID:12205100, PMID:18417535, PMID:19303847
• RAD51 can peel nucleosomal DNA from histones, facilitating repair in chromatin. Related references: PMID:38509361
• It is recruited to stalled replication forks to promote fork restart and prevent collapse. Related references: PMID:27797818, PMID:31844045
• Additionally, RAD51 functions in mitochondrial DNA maintenance under oxidative stress and participates in interstrand cross-link repair. Related references: PMID:20413593, PMID:26253028
• The protein localizes predominantly to the nucleus and chromosomes, but also to the cytoplasm, centrosome, and mitochondrial matrix.
• RAD51 is highly expressed in testis, thymus, and other proliferative tissues, with lower levels in breast. Related references: PMID:18417535

Experimental Guidance and Technical Tips

• The immunogen spans full-length human RAD51 (aa 1-339), so the polyclonal antibody likely recognizes multiple epitopes and may detect various isoforms.
• For Western blot, a band around 37 kDa is expected; consider using reducing conditions and validate with appropriate positive and negative cell lysates.
• For IHC-P, antigen retrieval may be necessary; test different retrieval buffers and antibody dilutions on known positive tissues (e.g., testis, thymus).
• For ELISA, use the recombinant protein or cell lysates as coating antigen; perform checkerboard titrations to determine optimal coating and detection antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

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