Anti-RAD51 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q06609

Core Product Specifications and Parameters

Biological Background: RAD51 Function and Localization

• Functions as a homologous DNA recombinase catalyzing strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:12205100, PubMed:12442171, PubMed:15937124). Related references: PMID:12205100, PMID:12442171, PMID:15937124
• Binds to single-stranded DNA (ssDNA) in an ATP-dependent manner to form nucleoprotein filaments essential for homology search and strand exchange (PubMed:12205100, PubMed:15937124, PubMed:17515904). Related references: PMID:12205100, PMID:15937124, PMID:17515904
• Catalyzes recognition of homology and strand exchange between homologous DNA partners to form joint molecules between processed DNA breaks and repair templates (PubMed:12205100, PubMed:18417535, PubMed:19303847). Related references: PMID:12205100, PMID:18417535, PMID:19303847
• Promotes removal of RPA from ssDNA and stabilizes RAD51-ssDNA filaments by blocking ATP hydrolysis (PubMed:20729859). Related references: PMID:20729859
• RAD51 filaments peel nucleosomal DNA from histone surfaces, enabling template access (PubMed:38509361). Related references: PMID:38509361
• Recruited to resolve stalled replication forks during replication stress (PubMed:27797818, PubMed:31844045). Related references: PMID:27797818, PMID:31844045
• Acts as an accessory factor for DMC1 recombinase during meiosis, enabling proper chromosome pairing and crossing over (By similarity).
• Involved in interstrand cross-link repair (PubMed:26253028) and regulation of mitochondrial DNA copy number under oxidative stress with RAD51C and XRCC3 (PubMed:20413593). Related references: PMID:26253028, PMID:20413593
• Highly expressed in testis and thymus; weakly in breast (PubMed:18417535). Localizes to nucleus, chromosomes, cytoplasm, perinuclear region, mitochondrion matrix, and centrosome. Related references: PMID:18417535
• Subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, regulating its activity; associated with Fanconi anemia and cancer.

Experimental Guidance and Technical Tips

• The immunogen encompasses full-length human RAD51 (aa 1-339), offering broad epitope coverage; consider this for applications requiring native conformation.
• For Western blotting, expect a band near 37 kDa; validate with positive control lysates (e.g., testis or thymus) and appropriate loading controls.
• In IHC-P, optimize antigen retrieval and consider tissue expression patterns; testis or tumor samples may show strong nuclear staining.
• For IF/ICC, RAD51 forms distinct nuclear foci after DNA damage; include a positive control with induced double-strand breaks (e.g., ionizing radiation).
• For IP and ELISA, the antibody may capture endogenous RAD51 or recombinant proteins; validate with known interaction partners.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing. Our anti-RAD51 rabbit monoclonal antibody supports your DNA damage repair, homologous recombination, and genomic stability research, bridging the gap from concept to clinical application. Contact us for tailored solutions and technical consultation.