Anti-Rad51 Rabbit Monoclonal Antibody for WB, ELISA, ChIP - Q06609

Core Product Specifications and Parameters

Biological Background: DNA repair protein RAD51 homolog 1 (RAD51) Function and Localization

• RAD51 catalyzes homologous DNA strand exchange, a central step in DNA repair through homologous recombination (HR), by forming ATP-dependent nucleoprotein filaments on single-stranded DNA (ssDNA). Related references: PMID:12205100, PMID:12442171, PMID:15937124, PMID:17515904
• It recognizes homology between homologous DNA partners and catalyzes strand exchange to form joint molecules, linking processed DNA breaks with repair templates. Related references: PMID:12205100, PMID:18417535, PMID:19303847
• RAD51 is recruited to stalled replication forks to resolve replication stress and participates in interstrand cross-link repair. Related references: PMID:27797818, PMID:31844045, PMID:26253028
• Under oxidative stress, RAD51 cooperates with RAD51C and XRCC3 to regulate mitochondrial DNA copy number. Related references: PMID:20413593
• During meiosis, RAD51 serves as a non-catalytic accessory factor for DMC1 recombinase, facilitating chromosome pairing and crossing over.
• RAD51 dynamically localizes to the nucleus, chromosomes, cytoplasm, perinuclear region, mitochondrial matrix, and centrosome, reflecting its multifaceted roles in DNA metabolism.
• RAD51 is highly expressed in testis and thymus, with moderate expression in small intestine, placenta, colon, pancreas, and ovary, and weak expression in breast. Related references: PMID:18417535
• RAD51 undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation, which regulate its activity and protein interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-100) of human RAD51; this epitope is conserved in mouse and rat, which may explain the observed cross-reactivity.
• For Western blot (WB), use reducing and denaturing conditions; expect a band around 37 kDa. Optimize antibody dilution and detection conditions for your specific sample type.
• For ELISA, consider pairing with a detection antibody against a non-overlapping epitope; validate with recombinant RAD51 or cell lysates.
• In ChIP assays, use appropriate crosslinking (e.g., formaldehyde) and optimize sonication; RAD51 enrichment can be induced by DNA-damaging agents (e.g., etoposide).
• This antibody has been reported to react with human, mouse, and rat; for other species, verify sequence homology of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw material supplier, CamelBio supports diagnostic manufacturers with high-quality antibodies for DNA repair and oncology biomarkers, including validated antibody pairs and optimized monoclonal reagents. Our catalog features ready-to-use products like this RAD51 monoclonal antibody, and we can rapidly source rare-target raw materials for custom assay development. From concept to clinic, CamelBio streamlines your procurement and accelerates diagnostic innovation.