Anti-Rad50 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q92878

Core Product Specifications and Parameters

Biological Background: RAD50 Function and Localization

• RAD50 is a core component of the MRN complex (MRE11-RAD50-NBS1), and it plays a central role in DNA double-strand break repair, homologous recombination, telomere integrity, and meiosis.
  Related references: PMID:15064416, PMID:21757780, PMID:27889449
• The MRN complex mediates DNA end resection, generating single-stranded DNA critical for homologous recombination, and it facilitates the recruitment of repair factors and activation of ATM/ATR kinases after DNA damage.
  Related references: PMID:15064416, PMID:27889449, PMID:28867292, PMID:15790808, PMID:16622404
• The complex possesses single-strand endonuclease and double-strand 3′-5′ exonuclease activities (provided by MRE11), while RAD50 binds and tethers DNA ends to regulate MRE11 activity.
  Related references: PMID:11741547, PMID:9590181, PMID:9651580, PMID:12805565, PMID:28134932
• RAD50 contains ATP-binding domains; ATP binding induces a closed conformation that optimally positions DNA ends within the MRE11 active site (by similarity).
• RAD50 is predominantly nuclear, localizing to chromosome telomeres and sites of DNA damage; it also participates in processing R-loops and modulating t-loop formation.
  Related references: PMID:10888888, PMID:31537797
• RAD50 is expressed at low levels in most tissues, with elevated expression in testis and fibroblasts.
• RAD50 is subject to acetylation and phosphorylation, and is characterized by ATP-binding, coiled-coil, zinc-binding, and hydrolase motifs.

Experimental Guidance and Technical Tips

• For Western blot analysis, expect a band near 154 kDa. Use an appropriate positive control lysate from human, mouse, or rat cells and include a loading control.
• For IHC-P on paraffin-embedded sections, standard antigen retrieval methods (e.g., heat-induced epitope retrieval) may be considered; pilot experiments are recommended.
• For immunoprecipitation (IP), use standard protocols with pre-clearing steps; the antibody is suitable for pulling down endogenous RAD50 complexes from nuclear extracts.
• For ELISA, the peptide immunogen (N-terminal residues 1-100) may be used to validate antibody titer; cross-reactivity with closely related proteins should be ruled out by sequence alignment.
• Due to its cross-species reactivity, this antibody can be employed for comparative studies in human, mouse, and rat models of DNA repair.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner for diagnostic manufacturers, labs, and research institutes. Our RAD50 rabbit polyclonal antibody is ideal for investigating DNA repair pathways and genomic instability, and we can further support your projects with validated monoclonal/polyclonal antibody pairs, optimized bulk ancillary reagents, and assistance in sourcing rare-target raw materials. Whatever your diagnostic or research development needs—from concept to clinic—CamelBio streamlines your supply chain.