Anti-Rad50 Polyclonal Antibody for WB, IHC-P, ELISA - Q92878

Core Product Specifications and Parameters

Biological Background: Rad50 Function and Localization

• RAD50 is a component of the MRN complex (together with MRE11 and NBN), which plays a central role in double-strand break (DSB) repair, DNA recombination, and maintenance of telomere integrity (PubMed:15064416, PubMed:21757780, PubMed:27889449). Related references: PMID:15064416 PMID:21757780 PMID:27889449
• The MRN complex is essential for the repair of DNA double-strand breaks via homologous recombination (HR), an error-free mechanism that primarily occurs during S and G2 phases (PubMed:15064416, PubMed:21757780). Related references: PMID:15064416 PMID:21757780
• RAD50 contributes to DNA end resection by binding DNA ends and regulating MRE11 exonuclease/endonuclease activities; ATP binding induces a conformational switch that controls MRE11 nuclease accessibility (PubMed:11741547, PubMed:12805565, PubMed:28134932). Related references: PMID:11741547 PMID:12805565 PMID:28134932
• The MRN complex also mediates DNA damage signaling by activating ATM and ATR kinases, a function independent of MRE11 nuclease activity (PubMed:15064416, PubMed:15790808, PubMed:16622404). Related references: PMID:15064416 PMID:15790808 PMID:16622404
• RAD50 localizes to the nucleus, and is found at chromosome telomeres where it may modulate t-loop formation (PubMed:10888888). Related references: PMID:10888888
• Expression of RAD50 is very low in most tissues, with higher levels observed in testis and fibroblasts.
• RAD50 is subject to post-translational modifications including acetylation and phosphorylation, and features ATP-binding, coiled-coil, and zinc-binding domains (UniProt keywords).
• The MRN complex also processes R-loops, contributing to genomic stability (PubMed:31537797). Related references: PMID:31537797

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-270) of human RAD50; therefore, the antibody is expected to recognize the N-terminus of the protein.
• For western blot (WB), RAD50 migrates at ~154 kDa; ensure proper SDS-PAGE separation and transfer conditions (e.g., extended transfer times) for high-molecular-weight proteins.
• For immunohistochemistry (IHC-P), validate staining in human testis or fibroblast samples, which exhibit higher RAD50 expression; consider antigen retrieval optimization.
• For ELISA, the synthetic peptide immunogen can serve as a positive control to confirm antibody reactivity; performance on endogenous full-length protein should be validated independently.
• Given the central role of RAD50 in DNA repair and telomere biology, this antibody may assist in chromatin immunoprecipitation (ChIP) or DNA damage foci assays, though such applications have not been validated with this product and require user optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing solutions, from validated antibody pairs to bulk ancillary reagents, streamlining your diagnostic development pipeline. For researchers focused on DNA repair, genome stability, and oncology, our portfolio includes high-quality polyclonal antibodies like this anti-RAD50 reagent, ready to support immunoassay optimization and biomarker discovery. Whether you require rare-target antibodies, recombinant proteins, or custom conjugation services, CamelBio bridges the gap between concept and clinic with efficient, reliable supply.