Anti-Rad23B Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P54727

Core Product Specifications and Parameters

Biological Background: Rad23B Function and Localization

• Multifunctional protein that participates in histone H4K20 demethylation, DNA repair, ubiquitin-dependent protein degradation and transcriptional regulation. Related references: PMID:10488153, PMID:32209475, PMID:9372924
• Specifically demethylates mono-, di- and trimethylated 'Lys-20' of histone H4 (H4K20me1, H4K20me2, H4K20me3) into unmethylated forms; activates transcription of coding genes by demethylating H4K20me1 and repetitive elements by demethylating H4K20me3. Related references: PMID:32209475
• Acts as a multiubiquitin chain receptor that binds polyubiquitin chains and delivers ubiquitinated substrates to the 26S proteasome for degradation. Related references: PMID:10488153, PMID:19435460
• May facilitate endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by associating with PNGase and delivering deglycosylated proteins to the proteasome. Related references: PMID:15358861
• Component of the XPC complex in global genome nucleotide excision repair (GG-NER); the XPC:RAD23B dimer binds to UV- and cisplatin-damaged DNA and stabilizes XPC. Related references: PMID:10873465, PMID:12815074, PMID:9372924
• Subcellular localization: found in both nucleus and cytoplasm.
• Tissue specificity: highly expressed in testis and ejaculated spermatozoa.
• Alternative splicing produces multiple isoforms; classified as a phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100–200 of human Rad23B; cross-reactivity with mouse and rat suggests epitope conservation in this region.
• For Western blotting, expect a band at approximately 43 kDa; validate specificity in relevant cell or tissue lysates.
• Suitable for IHC-P, IF/ICC, and ELISA; optimize dilutions and conditions for each application in the intended sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal antibodies like this anti-Rad23B reagent, and bulk ancillary supplies to support DNA repair and ubiquitin-proteasome research. From rare targets to routine production, we bridge the gap between concept and clinic with reliable raw materials and technical support.