Anti-Rad23A Rabbit Polyclonal Antibody for WB, ELISA - P54725

Core Product Specifications and Parameters

Biological Background: Rad23A Function and Localization

• RAD23A (HR23A) is a multifunctional protein that participates in histone H4K20 demethylation, DNA repair, ubiquitin-dependent protein degradation, transcriptional regulation, and viral replication.
  Related references: PMID:12643283 PMID:14621999 PMID:15321727
• It specifically demethylates mono-, di-, and trimethylated lysine 20 of histone H4 (H4K20me1, H4K20me2, H4K20me3), thereby regulating coding gene transcription (via H4K20me1) and repetitive element transcription (via H4K20me3).
  Related references: PMID:32209475
• RAD23A plays a critical role in global genome nucleotide excision repair (GG-NER) by forming a complex with XPC that recognizes DNA damage.
  Related references: PMID:9372924
• It binds to 'Lys-48'-linked polyubiquitin chains and, with lower affinity, to 'Lys-63'-linked chains, delivering ubiquitinated substrates to the 26S proteasome for degradation.
  Related references: PMID:12643283 PMID:14621999 PMID:15321727
• Located primarily in the nucleus, RAD23A undergoes alternative splicing and is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation.
• During HIV-1 infection, RAD23A facilitates viral replication in non-proliferating cells and primary macrophages by mediating the association of Vpr with the host proteasome.
• The predicted molecular weight of RAD23A is approximately 40 kDa (calculated 39,609 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human RAD23A (aa 1–363), providing broad recognition potential; confirm specificity in your experimental system.
• For Western blotting, use a standard protocol with a polyclonal antibody; the expected band appears near 40 kDa. Optimize dilution based on endogenous expression levels.
• For ELISA, validate the antibody with recombinant RAD23A or peptide antigens to establish sensitivity and linearity.
• Cross-reactivity is documented for human and mouse samples; activity in other species should be verified if required.
• Consider using protease inhibitors during sample preparation, as RAD23A may be susceptible to degradation.
• The antibody may detect splice variants or modified forms; confirm identity by mass spectrometry or knockdown controls where needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to validated IVD raw materials, technical services, and consulting—from early development through clinical readiness. This anti-RAD23A rabbit polyclonal antibody supports research in DNA repair, chromatin biology, and ubiquitin-proteasome pathways. We offer reliable supply of high-quality antibodies, bulk ancillary reagents, and sourcing capabilities for rare and custom targets to accelerate your assay development.