Anti-Rad23A Monoclonal Antibody for WB, IF/ICC, ELISA - P54725

Core Product Specifications and Parameters

Biological Background: Rad23A Function and Localization

• RAD23A, also known as UV excision repair protein RAD23 homolog A (HR23A/hHR23A), is a multifunctional protein that participates in histone H4K20 demethylation, DNA repair, ubiquitin-dependent protein degradation, transcriptional regulation, and viral replication. Related references: PMID:12643283 PMID:14621999 PMID:15321727
• It specifically demethylates mono-, di- and trimethylated 'Lys-20' of histone H4 (H4K20me1, H4K20me2, H4K20me3) into unmethylated forms, which activates transcription of coding genes and repetitive elements. Related references: PMID:32209475
• RAD23A is crucial for nucleotide excision repair (NER): it forms a complex with XPC that demonstrates NER activity in vitro, and it stabilizes XPC. Related references: PMID:9372924
• It facilitates ubiquitin-dependent protein degradation by binding 'Lys-48'-linked polyubiquitin chains and delivering ubiquitinated substrates to the 26S proteasome. Related references: PMID:12643283 PMID:14621999 PMID:15321727
• RAD23A plays a role in HIV-1 replication: it is required for the association of HIV-1 Vpr with the host proteasome in non-proliferating cells and primary macrophages.
• Subcellular localization: predominantly nuclear.
• The protein undergoes alternative splicing and phosphorylation, and contains ubiquitin-like (Ubl) domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 200–300) of human RAD23A. The antibody may detect full-length RAD23A and potentially isoforms containing this region. Validate specificity in the relevant sample system.
• For Western blotting, a band around 40 kDa is expected. Optimize loading controls and antibody dilution for your specific lysate.
• In immunofluorescence/ICC, expect nuclear staining consistent with RAD23A localization. Use appropriate fixation and permeabilization protocols.
• For ELISA, consider coating conditions with the synthetic peptide or recombinant protein to assess binding. Perform cross-reactivity tests if applied to non-human/mouse species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw-material procurement for diagnostic manufacturers, labs, and research institutes. For studies on DNA repair, histone modification, or the ubiquitin-proteasome pathway, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. As a one-stop bridge, CamelBio connects you with the precise raw materials needed from concept to clinic.