Anti-Rad21 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA, ChIP - O60216

Core Product Specifications and Parameters

Biological Background: Rad21 Function and Localization

• Rad21 (SCC1 homolog, NXP1) is a core component of the cohesin complex, critical for sister chromatid cohesion from DNA replication in S phase until their segregation in mitosis, ensuring proper chromosome segregation and post-replicative DNA repair. Related references: PMID:11509732
• The cohesin complex, including Rad21, is implicated in spindle pole assembly during mitosis. Related references: PMID:11590136
• In interphase, Rad21 functions in transcriptional regulation by binding to numerous genomic sites; specifically, it binds to and represses the APOB gene promoter. Related references: PMID:25575569
• Rad21 may also control RUNX1 gene expression and play a role in embryonic gut development, potentially via regulation of enteric neuron development.
• Subcellular localization includes nucleus, nuclear matrix, centromeric chromatin, spindle pole, and cytosol, reflecting its dynamic roles during the cell cycle.
• Tissue-specific expression: Rad21 protein is detected in the gut.
• Rad21 is a phosphoprotein and undergoes ubiquitination, with functional involvement in apoptosis, DNA repair, and transcriptional activation/repression.

Experimental Guidance and Technical Tips

• For Western blot, a band around 72 kDa (calculated molecular weight) is anticipated; validate the antibody in human, mouse, or rat cell lysates (e.g., HeLa, NIH/3T3) based on reported cross-reactivity.
• In chromatin immunoprecipitation (ChIP) assays, Rad21 is a chromatin-bound cohesin subunit; consider optimizing cross-linking (e.g., formaldehyde) and chromatin shearing to enrich target genomic regions.
• For immunohistochemistry (IHC-P), antigen retrieval with heat-induced epitope retrieval (HIER) may be necessary; positive staining is expected in gut tissues, reflecting endogenous expression.
• For immunofluorescence (IF/ICC), Rad21 typically shows nuclear and centromeric localization; include a nuclear counterstain (DAPI) and validate in the appropriate cell model.
• As a rabbit polyclonal antibody, batch-to-batch consistency should be verified for quantitative applications; consider using a verified loading control or normalization standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing for IVD raw materials and technical support. This anti-Rad21 polyclonal antibody is part of our catalog for chromatin and cell division research applications. We supply validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents to streamline your assay development and manufacturing workflows.