Anti-Rad21 Rabbit Monoclonal Antibody for WB, ELISA, ChIP, ChIP-seq - O60216

Core Product Specifications and Parameters

Biological Background: Rad21 Function and Localization

• As a member of the cohesin complex, Rad21 is essential for sister chromatid cohesion from S phase through mitosis, ensuring proper chromosome segregation and post-replicative DNA repair; it also prevents inappropriate recombination between repetitive regions (PubMed:11509732). Related references: PMID:11509732

• The cohesin complex, including Rad21, may also play a role in spindle pole assembly during mitosis (PubMed:11590136). Related references: PMID:11590136

• In interphase, Rad21 regulates gene expression by binding to numerous genomic sites; it may control RUNX1 expression and directly represses the APOB gene promoter (PubMed:25575569). Related references: PMID:25575569

• Rad21 may promote apoptosis, contributing to programmed cell death.

• Subcellularly, Rad21 localizes to the nucleus, chromatin, centromeres, and spindle poles, with presence also detected in the cytoplasm and cytoskeleton.

• Tissue-specific expression includes the gut, where Rad21 may be involved in embryonic gut development and enteric neuron development.

• Post-translational modifications and key features: Rad21 is a phosphoprotein, undergoes ubiquitin-like conjugation, and possesses DNA-binding activity.

Experimental Guidance and Technical Tips

• The immunogen targets a C-terminal region (AA 532–631); the antibody may detect full-length Rad21 and potentially C-terminal fragments. Validate specificity in your sample system.
• For WB, consider a 72 kDa expected band; optimize loading controls and blocking conditions for reliable detection.
• As a chromatin-associated protein, ChIP and ChIP-seq applications may require optimized crosslinking, shearing, and antibody amounts. Include appropriate negative controls.
• The recombinant monoclonal nature ensures batch-to-batch reproducibility; still verify performance under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, including this anti-Rad21 monoclonal antibody for target validation, diagnostic development, and research into cohesin biology, chromosome dynamics, and gene regulation. Our portfolio extends to validated antibody pairs, recombinant antigens, and bulk ancillary reagents, supporting every stage from concept to clinic. Whether you need standard products or rare-target sourcing, CamelBio bridges the gap for your development pipeline.