Anti-RAD18 Rabbit Polyclonal Antibody for WB, ELISA - Q9NS91

Core Product Specifications and Parameters

Biological Background: RAD18 Function and Localization

• RAD18 is an E3 ubiquitin-protein ligase that plays a key role in postreplication repair of UV-damaged DNA by filling gaps in daughter strands during replication of damaged DNA.
• Functions as part of the UBE2B-RAD18 ubiquitin ligase complex, which catalyzes the mono-ubiquitination of DNA-associated PCNA on Lys-164, a critical step for DNA damage tolerance.
• Exhibits single-stranded DNA (ssDNA) binding activity, suggesting a direct role in recognizing DNA lesions or replication fork structures.
• Localizes predominantly to the nucleus, with additional presence in the cytoplasm and at the centrosome, indicating potential roles beyond DNA repair.
• Post-translational modifications include phosphorylation and acetylation, which may regulate its activity or localization.
• Belongs to the RING finger protein family (RNF73) and contains a zinc finger domain essential for its ubiquitin ligase function.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 216-495, covering a substantial portion of the RAD18 protein; consider this when mapping binding epitopes.
• For Western blot, the predicted molecular weight is ~56 kDa; validate the observed band in the relevant sample system (e.g., human cell lines), and be aware of potential post-translational modifications that may shift the migration.
• In ELISA applications, ensure the coating antigen or capture format corresponds to the immunogen region to maintain reactivity.
• This antibody is polyclonal and may exhibit batch-to-batch variation; we recommend titrating the antibody for your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers by offering ready-to-use and custom-sourced raw materials, including this Rabbit anti-RAD18 polyclonal antibody. For DNA damage and repair research, we provide validated antibody pairs, optimized monoclonals and polyclonals, and ancillary reagents to streamline your assay development. Let our team help you source rare or niche targets efficiently.