Anti-Rad18 Monoclonal Antibody for WB and ELISA - Q9NS91

Core Product Specifications and Parameters

Biological Background: Rad18 Function and Localization

• RAD18 (also known as RNF73, hHR18, hRAD18) is an E3 ubiquitin-protein ligase belonging to the RING finger protein family. It contains a RING-type zinc finger domain essential for its ubiquitin transferase activity.
• RAD18 plays a critical role in postreplication repair of UV-damaged DNA by mediating gap-filling on the daughter strand during replication of damaged DNA.
• It forms a complex with the E2 ubiquitin-conjugating enzyme UBE2B to catalyze the mono-ubiquitination of DNA-associated proliferating cell nuclear antigen (PCNA) on Lys-164.
• RAD18 possesses single-stranded DNA (ssDNA) binding activity, which may facilitate its recruitment to sites of DNA damage.
• Subcellularly, RAD18 is primarily localized in the nucleus but can also be found in the cytoplasm, and at the centrosome within the microtubule organizing center.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, and it forms isopeptide bonds as part of its ubiquitination mechanism.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning amino acids 1-100 of human RAD18 (Q9NS91), which covers the N-terminal region. For Western blot (WB), an expected band at approximately 56 kDa should be observed; consider using appropriate loading controls and validate detection in your specific sample matrix.
• Due to its reactivity with human, mouse, and rat, this antibody may be suitable for cross-species studies, but we recommend verifying specificity in the relevant species via positive and negative controls.
• For ELISA applications, the peptide immunogen can serve as a coating antigen for assay development; optimize coating concentration and antibody dilution for best signal-to-noise ratio.
• No experimental dilution recommendations are provided; researchers should titrate the antibody for their specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, single-vendor solution for sourcing high-quality IVD raw materials, including validated monoclonal antibodies targeting DNA damage repair proteins like RAD18. Beyond this anti-RAD18 antibody, we supply antibody pairs, ancillary reagents, and custom sourcing for rare targets to support your assay development from concept to clinic. Contact us to discuss your specific procurement needs for postreplication repair and ubiquitination research tools.