Anti-RACK1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P63244

Core Product Specifications and Parameters

Biological Background: RACK1 Function and Localization

• RACK1 (Receptor for Activated C Kinase 1), also known as GNB2L1, HLC-7, or PIG21, is a scaffolding protein and an integral component of the 40S ribosomal subunit. It contains multiple WD repeat domains that mediate diverse protein–protein interactions.
• As part of the ribosome, RACK1 is involved in translational repression (PubMed:23636399) and initiates ribosome quality control (RQC) by promoting ubiquitination of 40S subunits during stalled translation (PubMed:28132843). Related references: PMID:23636399 PMID:28132843
• It binds and stabilizes activated protein kinase C (PKC), enhancing PKC-mediated phosphorylation, and may recruit PKC to the ribosome to phosphorylate EIF6, thereby regulating translation.
• RACK1 modulates a wide array of signaling pathways and cellular processes, including cell cycle progression (prolongs G0/G1 phase), apoptosis (promotes BAX oligomerization), integrin signaling, and Wnt pathway inhibition.
• Subcellularly, RACK1 is found in the cytoplasm, cell membrane, nucleus, perinuclear region, dendrites, and phagocytic cups, reflecting its multifunctional roles.
• In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells, and is upregulated in hepatocellular carcinomas, implicating it in hepatic fibrosis and cancer.
• Post‑translational modifications include phosphorylation and acetylation. RACK1 also participates in host–virus interactions, such as enhancing HIV‑1 Nef phosphorylation and remodeling ribosomes for poxvirus mRNA translation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–290 of human RACK1, covering the majority of the protein including several WD repeats. This design is expected to generate antibodies that recognize both native and denatured conformations.
• For Western blotting, the predicted molecular weight is approximately 35 kDa. Use appropriate SDS‑PAGE conditions and include a suitable loading control.
• In IF/ICC experiments, RACK1 exhibits cytoplasmic, membrane, and nuclear localization. Consider optimizing permeabilization and fixation conditions to detect all pools.
• When performing ELISA, the antibody can be used as capture or detection reagent. Validate its performance with purified RACK1 protein or relevant cell lysates in your assay setup.
• The antibody cross‑reacts with mouse and rat RACK1, making it suitable for rodent model studies. Confirm species‑specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw‑material supply, supporting diagnostic developers and research laboratories from early concept to clinical application. Our anti‑RACK1 rabbit polyclonal antibody is ideal for signaling, translation control, and oncology‑focused assay development. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and dedicated sourcing for rare‑target raw materials to streamline your workflow.