Anti-RAC3 Rabbit Polyclonal Antibody for WB, ELISA - P60763

Core Product Specifications and Parameters

Biological Background: RAC3 Function and Localization

• RAC3, also known as p21-Rac3, is a Ras-related C3 botulinum toxin substrate 3, a small GTPase.
• It cycles between active GTP-bound and inactive GDP-bound states, acting as a molecular switch.
• In its active state, RAC3 binds effector proteins to regulate cell spreading and the formation of actin-based protrusions such as lamellipodia and membrane ruffles.
• RAC3 promotes cell adhesion and spreading on fibrinogen via CIB1 and alpha-IIb/beta3 integrin-mediated pathways.
• Subcellularly, RAC3 localizes to the plasma membrane, cytoplasm, endomembrane system, lamellipodia, perinuclear region, and cytoskeleton.
• It contains characteristic motifs for GTP binding, magnesium and metal binding, and is subject to prenylation, methylation, and ubiquitin-like conjugation.
• RAC3 is predominantly expressed in brain, with detectable levels in heart, placenta, and pancreas.
• Structurally, the human RAC3 protein has a molecular weight of approximately 21.4 kDa (21379 Da) and is classified under glycoprotein, lipoprotein, and hydrolase families.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-192) of human RAC3, which includes the GTP-binding domain, making this antibody suitable for detecting both GTP- and GDP-bound forms.
• For Western blot (WB), a band around 21 kDa is expected; validate specificity using knockout or knockdown samples.
• For ELISA, consider pairing with a capture antibody targeting a distinct epitope for sandwich ELISA development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focused on GTPase signaling and cytoskeletal dynamics, we provide easy access to RAC3-targeting antibodies and related reagents to accelerate assay development. Our global supply network ensures reliable procurement of even rare targets, supporting diagnostic manufacturers from concept to clinic.