Anti-RAC2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P15153

Core Product Specifications and Parameters

Biological Background: RAC2 Function and Localization

• Official name: Ras-related C3 botulinum toxin substrate 2. Also known as GX, small G protein, and p21-Rac2.
• Functions as a plasma membrane-associated small GTPase, cycling between an active GTP-bound and inactive GDP-bound state to regulate cellular responses. Related references: PMID:30723080
• Acts as a regulatory subunit of the phagocyte NADPH oxidase complex, mediating the transfer of electrons from NADPH to O2 to produce superoxide (O2(-)). Related references: PMID:1660188
• Activates PLCG2, promoting signal transduction pathways involved in calcium mobilization and immune cell activation. Related references: PMID:19394299
• Participates in diverse biological processes including secretory processes, phagocytosis of apoptotic cells, and epithelial cell polarization.
• Subcellular localization: Predominantly cytoplasmic.
• Tissue specificity: Exclusively expressed in hematopoietic cells.
• Post-translational modifications include acetylation, ADP-ribosylation, glycosylation, prenylation, and methylation, which modulate its activity and localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human RAC2 protein (aa 1-192). It may detect both endogenous and recombinant RAC2; however, validation in the relevant sample system is recommended.
• For Western blotting, expect a band at approximately 21 kDa. Optimize blocking and antibody dilution for your specific samples and conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider using hematopoietic tissues or cell lines due to the hematopoietic-specific expression of RAC2.
• As a polyclonal antibody, it may cross-react with closely related RAC family members (e.g., RAC1, RAC3). Verify specificity using knockout controls or isoform-specific assays if necessary.
• The antibody is suitable for ELISA applications; pair with appropriate capture/detection antibodies for sandwich ELISA format after thorough validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw-material sourcing, supporting researchers and manufacturers in the fields of small GTPase signaling, NADPH oxidase biology, and hematopoiesis. Our supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials, facilitating seamless transition from concept to clinic. Whether you need individual antibodies or integrated reagent sets for diagnostic development, CamelBio is your trusted partner for reliable supply and technical consultation.