Anti-Rac1/2/3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P63000 / P15153 / P60763

Core Product Specifications and Parameters

Biological Background: Rac1/2/3 Function and Localization

• Rac1, also known as p21-Rac1, TC-25, or MIG5, is a member of the Rho family of small GTPases. Alternative splicing produces isoform B, which is predominantly expressed in skin and intestinal epithelial tissues and is frequently elevated in colorectal tumors.
• Acts as a molecular switch cycling between active GTP-bound and inactive GDP-bound states, regulating diverse processes including phagocytosis, cell migration, membrane ruffling, and secretory pathways. Related references: PMID:1643658, PMID:22843693, PMID:23512198
• Upon activation, binds effectors such as PAK and PKN2, mediating actin cytoskeleton reorganization, cell adhesion assembly/disassembly (via DSG3), and growth-factor induced signaling. Related references: PMID:9121475, PMID:12695502, PMID:22796473
• Essential subunit of the phagocyte NADPH oxidase complex, which produces superoxide anions for microbial killing. Related references: PMID:38355798
• Localizes to the plasma membrane, lamellipodia, ruffles, dendrites, synapses, and nucleus, supporting its roles in cell motility, synaptic plasticity, and nuclear signaling.
• Post-translational modifications include prenylation, palmitoylation, phosphorylation, methylation, and ubiquitination, which dynamically regulate its membrane targeting, activity, and protein stability.
• Isoform B exhibits accelerated GEF-independent GDP/GTP exchange and impaired GTP hydrolysis, partially restored by GTPase-activating proteins (GAPs), and is associated with colorectal neoplasia. Related references: PMID:14625275

Experimental Guidance and Technical Tips

• This polyclonal antibody is raised against a recombinant protein corresponding to amino acids 1-192 common to Rac1, Rac2, and Rac3, enabling pan-Rac detection.
• For Western blot (WB), a band around 21 kDa is expected; consider using whole-cell lysates from human, mouse, or rat cells and titrating the antibody dilution to achieve optimal signal-to-noise ratio.
• In immunohistochemistry (IHC-P), antigen retrieval may improve staining; validate with appropriate positive and negative control tissues.
• For immunofluorescence (IF/ICC), fix cells with paraformaldehyde and permeabilize; Rac1/2/3 localizes to membrane ruffles, lamellipodia, and cytoplasmic compartments.
• When using in ELISA, ensure the recombinant antigen or native target is accessible; the polyclonal nature may provide robust signal amplification.
• As Rac proteins are highly conserved across species, cross-reactivity with human, mouse, and rat is expected; however, verify expression levels in the specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined procurement channel for high-quality IVD raw materials. This anti-Rac1/2/3 polyclonal antibody is part of our extensive catalog supporting cell migration, cancer biology, and neuroscience research. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal reagents, bulk ancillary supplies, and custom sourcing for rare targets to accelerate your assay development and production pipelines.