Anti-Rac GTPase-activating protein 1 (RGAP1) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H0H5

Core Product Specifications and Parameters

Biological Background: RGAP1 Function and Localization

• Rac GTPase-activating protein 1 (RGAP1), also known as MgcRacGAP or CYK4, is a core component of the centralspindlin complex essential for cytokinesis. It mediates microtubule-dependent and Rho-driven signaling that leads to myosin contractile ring formation and subsequent cell division. Related references: PMID:18511905
• RGAP1 sequentially interacts with ECT2 and RAB11FIP3 to coordinate cleavage furrow ingression and abscission, ensuring proper midbody attachment to the cell membrane during the final stages of cytokinesis. Related references: PMID:18511905
• Beyond its role in cell division, RGAP1 regulates growth and differentiation of hematopoietic cells and plays a critical role in erythropoiesis. Related references: PMID:10979956, PMID:34818416
• The protein exhibits strong GTPase-activating activity toward CDC42 and RAC1, with weaker activity toward RHOA, highlighting its regulatory function in Rho family GTPase signaling networks.
• RGAP1 dynamically localizes to multiple subcellular compartments, including the nucleus, cytoplasm, spindle, cleavage furrow, midbody, and cell membrane, reflecting its multifaceted roles throughout the cell cycle.
• Tissue expression is highest in testis, thymus, and placenta, with cell cycle-dependent regulation peaking at G2/M phase; it also contributes to spermatogenesis and sulfate transport in male germ cells.
• The protein contains zinc-finger domains, coiled-coil regions, and lipid-binding motifs, and undergoes phosphorylation and acetylation, as indicated by structural and post-translational modification data.

Experimental Guidance and Technical Tips

• The immunogen spans residues 353–632 of human RGAP1, a region that may encompass functional domains; consider verifying coverage of any known splice variants or isoforms in your experimental setup.
• In Western blot, a predominant band near 71 kDa is expected across human, mouse, and rat samples. Validate specificity using appropriate positive/negative controls, such as knockout lysates or overexpression models.
• For immunohistochemistry (IHC-P), antigen retrieval steps may be required; optimize fixation and permeabilization protocols for the target tissue to achieve robust staining.
• Immunofluorescence (IF/ICC) can reveal RGAP1 localization at the spindle, midbody, and cleavage furrow in dividing cells; co-stain with cytoskeletal markers (e.g., α-tubulin) to confirm mitotic structures.
• ELISA-based detection may be suitable for quantifying RGAP1 in cell lysates or recombinant protein preparations. Include standard curves and blank controls to ensure assay linearity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting your cell cycle and cytokinesis research with this anti-RGAP1 polyclonal antibody. Our extensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized supply solutions for rare and emerging targets. We streamline your path from concept to clinic, ensuring you have the critical raw materials needed for assay development and diagnostics manufacturing.