Anti-RABGAP1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y3P9

Core Product Specifications and Parameters

Biological Background: RABGAP1 Function and Localization

• RABGAP1, also known as Rab GTPase-activating protein 1, GAPCenA (GAP and centrosome-associated protein), or TBC1D11, acts as a GTPase-activating protein (GAP) for RAB6A, promoting GTP hydrolysis to regulate Rab-mediated trafficking and signaling.
• It plays a role in microtubule nucleation at the centrosome and is implicated in the metaphase-anaphase transition during cell division, linking membrane trafficking with cell cycle control.
• RABGAP1 contains a TBC (Tre-2/Bub2/Cdc16) domain characteristic of RabGAPs, along with coiled-coil regions that may facilitate protein-protein interactions and centrosomal targeting.
• Localization is predominantly cytoplasmic, with enrichment at the centrosome and within the cytosol, as indicated by subcellular fractionation and immunofluorescence studies.
• The protein undergoes alternative splicing, producing multiple isoforms, and is regulated by phosphorylation, as evidenced by proteomics data and the presence of phosphosites in databases.
• Molecular weight is approximately 122 kDa (calculated ~121.7 kDa); the antibody recognizes a band near this size in Western blotting of appropriate lysates.
• RABGAP1 has been identified in high-throughput proteomic screens, confirming its expression across human tissues and its association with the centrosomal proteome.

Experimental Guidance and Technical Tips

• The immunogen was designed against a C-terminal region (aa 830-1069) of human RABGAP1, which spans part of the coiled-coil domain and may include regions critical for GAP activity; thus, the antibody may recognize functional epitopes.
• For Western blotting, use standard reducing conditions and load 20–50 µg of whole-cell lysate; the predicted band is at 122 kDa, and mouse cross-reactivity allows analysis in common cell lines such as NIH/3T3 or MEFs.
• In immunohistochemistry (IHC-P), antigen retrieval using citrate or EDTA buffer is recommended; optimize antibody dilution (e.g., 1:50–1:200) and include appropriate negative controls to confirm specificity.
• For ELISA, the antibody can be used as a capture or detection reagent; preliminary checkerboard titrations are advised to determine optimal coating and antibody concentrations.
• Because RABGAP1 is a low-abundance protein involved in cell cycle regulation, consider synchronizing cells to enrich for metaphase cells if assessing centrosomal localization or cell cycle-dependent expression changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, connecting diagnostic manufacturers and research labs with high-quality antibodies and reagents. This anti-RABGAP1 polyclonal antibody is part of our extensive portfolio for cell cycle and centrosome biology research. We support your assay development with validated antibody pairs, bulk reagents, and customized sourcing solutions for rare targets—helping you move from concept to clinic efficiently.