Anti-RABAC1 Polyclonal Antibody for WB, ELISA - Q9UI14

Core Product Specifications and Parameters

Biological Background: RABAC1 Function and Localization

• RABAC1, also known as PRA1 family protein 1, is a prenylated Rab acceptor protein that acts as a general Rab protein regulator required for vesicle formation from the Golgi complex.
• It may control vesicle docking and fusion by mediating the action of Rab GTPases on SNARE complexes, and it inhibits the removal of Rab GTPases from membranes by GDI.
• RABAC1 localizes to multiple cellular compartments, including the cell membrane, cytoplasm, Golgi apparatus, cytoplasmic vesicles, secretory vesicles, and synaptic vesicles.
• The protein is expressed ubiquitously, with strongest expression in placenta, pituitary gland, kidney, lung, and stomach.
• UniProt keywords associated with RABAC1 include transmembrane helix, synapse, and proteomics identification, reflecting its membrane association and synaptic localization.
• The molecular weight of the canonical isoform is approximately 20.6 kDa (UniProt), consistent with the observed ~21 kDa band.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal portion (amino acids 1–185), which includes the predicted transmembrane region; for detection of endogenous full-length protein, consider using membrane protein extraction protocols.
• In Western blotting, expect a band around 21 kDa; due to ubiquitous expression, recommended positive control lysates include placenta, kidney, lung, or stomach.
• For ELISA applications, optimize coating antigen concentration and consider using recombinant RABAC1 or peptide fragments for standard curves.
• Cross-reactivity with mouse and rat has been indicated; validate in your species of interest with appropriate positive and negative controls.
• As a polyclonal antibody, lot-to-lot consistency may vary; initial titration experiments are recommended for each new application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials, including validated antibodies for vesicle trafficking targets like RABAC1. Our portfolio features optimized polyclonal antibodies, recombinant proteins, and ancillary reagents to support your assay development. For specialized needs, our sourcing team can secure rare or custom raw materials to accelerate your project from concept to clinic.