Anti-RAB9A Monoclonal Antibody for WB, IF/ICC, ELISA - P51151

Core Product Specifications and Parameters

Biological Background: RAB9A Function and Localization

• RAB9A is a member of the Rab family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states to regulate intracellular membrane trafficking.
• Plays a critical role in the retrograde transport of proteins from late endosomes to the trans-Golgi network (TGN). Related references: PMID:34793709
• Utilizes NDE1/NDEL1 as an effector to interact with the dynein motor complex, facilitating the movement of Rab9-associated late endosomes toward the TGN. Related references: PMID:34793709
• Contributes to melanosome biogenesis by recruiting SGSM2 to melanosomes, ensuring proper trafficking of melanogenic enzymes TYR, TYRP1, and DCT/TYRP2.
• Localizes to multiple membrane compartments, including the cell membrane, endoplasmic reticulum, Golgi apparatus, late endosomes, phagosomes, cytoplasmic vesicles, and melanosomes.
• Post-translationally modified by acetylation, phosphorylation, and prenylation, which regulate its membrane association and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region of human RAB9A, which is conserved across human, mouse, and rat; this conservation likely underlies the observed cross-reactivity.
• For Western blotting, RAB9A runs at approximately 23 kDa; consider using appropriate loading controls and validating expression in relevant cell or tissue lysates.
• In immunofluorescence/ICC, RAB9A is expected to exhibit a punctate cytoplasmic staining pattern consistent with its endosomal, Golgi, and vesicular localization; co-staining with organelle markers may aid interpretation.
• For ELISA, this antibody may be suitable for detecting endogenous or recombinant RAB9A; optimal working concentrations should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive one-stop access to IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For investigations into intracellular trafficking, endosome-TGN dynamics, and melanosome biology, our RAB9A antibody and related reagents can streamline your assay development. We also specialize in rare target raw-material sourcing and provide technical consulting to help bridge the gap between concept and clinical application.