Anti-RAB8A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P61006

Core Product Specifications and Parameters

Biological Background: RAB8A Function and Localization

• RAB8A is a small GTPase that cycles between inactive GDP-bound and active GTP-bound conformations, functioning as a master regulator of intracellular membrane trafficking, polarized vesicular transport, and neurotransmitter release.
• Promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), driving apical surface formation and lumenogenesis in concert with RAB11A, the exocyst complex, and polarity proteins. Related references: PMID:20890297
• Controls the compacted morphology of the Golgi apparatus, thereby influencing Golgi organization and cargo processing. Related references: PMID:26209634
• Collaborates with MYO5B and RAB11A to establish epithelial cell polarization, essential for tissue architecture and function. Related references: PMID:21282656
• Participates in membrane trafficking to the primary cilium and is required for ciliogenesis, with implications for signaling and development. Related references: PMID:21844891 PMID:30398148 PMID:20631154
• Plays a role in autophagy, contributing to the sequestration and degradation of cytoplasmic components. Related references: PMID:27103069
• Upon lysosomal stress, RAB8A is recruited and stabilized via LRRK2-mediated phosphorylation, suppressing stress-induced lysosomal enlargement through EHBP1/EHNP1L1 effectors. Related references: PMID:30209220
• Exhibits broad subcellular localization including the plasma membrane, Golgi, endosomes, recycling endosomes, cilium, phagosomes, centrosome, midbody, and lysosomes, reflecting its versatile trafficking functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 140–207 of human RAB8A (NP_005361.2), a region likely to generate antibodies recognizing both full-length and possibly splice variants; verify specificity using relevant controls (e.g., siRNA knockdown or knockout lysates).
• For Western blot (WB), a band of approximately 24 kDa is expected based on the calculated molecular weight; use a suitable percentage SDS-PAGE gel for low-molecular-weight proteins and consider over-expression or enrichment for weak endogenous signals.
• For immunofluorescence/immunocytochemistry (IF/ICC), expect staining patterns consistent with RAB8A’s multifaceted localization (cytoplasmic vesicles, Golgi, plasma membrane, cilia); validate colocalization with organelle markers.
• For ELISA, ensure proper coating of the recombinant immunogen or native protein from cell/tissue lysates and perform titration to optimize signal-to-noise ratio.
• Cross-reactivity with mouse and rat RAB8A is specified; however, conservation of the epitope should be confirmed if working with other species or closely related RAB family members (e.g., RAB10, RAB13).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive, reliable access to IVD raw materials—from validated primary antibodies and recombinant proteins to ancillary reagents—streamlining the path from discovery to clinical application. For RAB8A-related investigations into intracellular trafficking, ciliopathies, autophagy, and cancer biology, our portfolio includes high-quality polyclonal antibodies like this RAB8A Rabbit pAb, as well as matched antibody pairs, bulk quantities, and customized sourcing for rare or specialized targets. Partner with CamelBio to accelerate your assay development and secure a consistent, scalable supply chain.