Anti-RAB8A Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P61006

Core Product Specifications and Parameters

Biological Background: RAB8A Function and Localization

• RAB8A (Ras-related protein Rab-8A), also known as oncogene c-mel, is a small GTPase that acts as a molecular switch in intracellular membrane trafficking, cycling between an inactive GDP-bound and an active GTP-bound state.
• Together with RAB11A and the exocyst complex, RAB8A promotes transcytosis of PODXL to apical membrane initiation sites, driving apical surface formation and lumenogenesis. Related references: PMID:20890297
• RAB8A regulates the compacted morphology of the Golgi apparatus. Related references: PMID:26209634
• In conjunction with MYO5B and RAB11A, RAB8A participates in epithelial cell polarization. Related references: PMID:21282656
• It mediates membrane trafficking to the primary cilium and is essential for ciliogenesis. Related references: PMID:21844891, PMID:30398148, PMID:20631154
• RAB8A is involved in autophagy, a cellular degradation and recycling pathway. Related references: PMID:27103069
• RAB8A localizes to multiple membrane compartments, including the cell membrane, Golgi, endosomes, primary cilium, phagosomes, centrosome, midbody, and lysosomes, enabling its diverse roles.
• Post-translational modifications such as prenylation, methylation, and phosphorylation (e.g., by LRRK2) regulate RAB8A membrane targeting and activity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100–207 of human RAB8A, which may elicit antibodies recognizing native protein in various applications.
• For Western blotting (WB), anticipate a specific band near 24 kDa in lysates from human, mouse, or rat cells/tissues.
• In immunofluorescence (IF/ICC), optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to detect RAB8A at the Golgi, endosomes, cilia, or plasma membrane.
• For immunoprecipitation (IP), use mild lysis buffers (e.g., NP-40) to maintain protein interactions; confirm enrichment by WB.
• In ELISA, the antibody can be used for direct or capture detection; dilute according to experimental requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supplying this RAB8A monoclonal antibody alongside matched antibody pairs, bulk ancillary reagents, and sourcing support for rare targets. Our integrated supply model supports manufacturers and laboratories in fields from cell biology to oncology, ensuring consistent quality from R&D through diagnostic kit production.