Anti-RAB7 Rabbit Polyclonal Antibody for WB, ELISA - P51149

Core Product Specifications and Parameters

Biological Background: RAB7 Function and Localization

• RAB7A, also known as Ras-related protein Rab-7a, is a small GTPase belonging to the Rab family, key regulators of intracellular membrane trafficking.
• Functions as a molecular switch cycling between inactive GDP-bound and active GTP-bound states to recruit effectors for vesicle formation, movement, tethering, and fusion. Related references: PMID:38538795
• Governs multiple steps of endo-lysosomal trafficking, including early-to-late endosomal maturation, endosomal migration along microtubules, and endosome-lysosome transport. Related references: PMID:11179213, PMID:12944476, PMID:14617358
• Critical for phagosome maturation and acidification, facilitating the engulfment and degradation of pathogens like S. aureus and M. tuberculosis.
• Plays a role in autophagy, lipolysis, and lipid metabolism, localizing to autophagosome and lipid droplet membranes (By similarity).
• Widely expressed with highest levels in skeletal muscle; involved in specialized pathways like melanosome maturation, neurotrophin transport, and osteoclast ruffled border formation.
• Mutations in RAB7A are associated with Charcot-Marie-Tooth disease type 2B (CMT2B), a peripheral neuropathy.
• Post-translational modifications include prenylation, phosphorylation, acetylation, and ubiquitination, which regulate its membrane targeting and function.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 23 kDa is expected for endogenous RAB7; validate with appropriate positive and negative controls.
• The polyclonal nature may help detect multiple epitopes, but lot-to-lot consistency should be verified.
• In ELISA, consider optimizing coating conditions and antibody dilutions against the target protein or peptide.
• Since cross-reactivity with mouse and rat is indicated, the antibody may be suitable for studies in these species; confirm reactivity in the relevant sample system.
• Given the immunogen design (peptide spanning amino acids 100-207), the antibody may recognize multiple isoforms; check sequence homology if isoform-specific detection is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-RAB7 antibody supports research and assay development in endo-lysosomal biology, autophagy, and neurodegenerative diseases like Charcot-Marie-Tooth. Whether you need large-scale production or specialized logistics, CamelBio bridges the gap from concept to clinic with reliable supply solutions.