Anti-RAB7 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P51149

Core Product Specifications and Parameters

Biological Background: RAB7 Function and Localization

• RAB7A is a small GTPase that cycles between inactive GDP-bound and active GTP-bound states, functioning as a key regulator of intracellular membrane trafficking from vesicle formation to fusion with target membranes. Related references: PMID:38538795
• In its active GTP-bound form, RAB7A recruits effectors to govern endo-lysosomal trafficking, including early-to-late endosome maturation, microtubule-dependent endosomal migration, and endosome-lysosome fusion. Related references: PMID:11179213, PMID:12944476, PMID:14617358
• Plays essential roles in growth factor receptor (e.g., EGF-EGFR) degradation, nutrient transporter uptake, neurotrophin transport in axons, and lipid metabolism through regulation of lipophagy.
• Required for the exosomal release of SDCBP, CD63, and syndecan, and for proper vesicular trafficking and cell surface expression of ACE2. Related references: PMID:22660413, PMID:33147445
• Involved in specialized membrane trafficking processes, including melanosome maturation, pathogen-induced phagosome and autophagosome maturation, and phagosome-lysosome fusion upon infection by S. aureus or M. tuberculosis.
• Subcellular localization: predominantly associated with late endosomes, lysosomes, phagosomes, autophagosomes, melanosomes, and lipid droplets; also detected on mitochondrial membranes under specific conditions.
• Post-translational modifications: prenylation for membrane anchoring; phosphorylation, acetylation, methylation, and isopeptide bond formation regulate activity and protein interactions.
• Widely expressed across tissues, with highest levels in skeletal muscle; linked to Charcot-Marie-Tooth disease type 2B (CMT2B) and host-virus interactions.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band migrating at approximately 23 kDa; consider including positive control lysates from human, mouse, or rat cell lines such as HeLa or skeletal muscle extracts.
• In immunofluorescence and immunoprecipitation experiments, optimize fixation/permeabilization conditions to preserve endosomal structures and antibody accessibility.
• The immunogen sequence (aa 108–207) is highly conserved across human, mouse, and rat, supporting cross-reactivity; validate specificity in your sample system with appropriate knockdown or knockout controls.
• For IHC-P, test standard antigen retrieval methods (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and antibody dilutions on known positive control tissues.
• This antibody may be suitable for ELISA-based detection; consider pairing with a matched detection antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers and research labs, offering validated monoclonal antibodies like this anti-RAB7 clone for endosomal trafficking and autophagy research. Beyond individual reagent supply, we provide optimized antibody pairs, bulk ancillary reagents, and rare-target sourcing services to accelerate your assay development from concept to clinic. Leverage our integrated supply chain to secure high-quality, application-tested antibodies for your next diagnostic project.