Anti-RAB7 Polyclonal Antibody for WB, IP, ELISA - P51149

Core Product Specifications and Parameters

Biological Background: RAB7 (Ras-related protein Rab-7a) Function and Localization

• RAB7A is a small GTPase of the Rab family, cycling between GDP-bound inactive and GTP-bound active forms to recruit effectors for vesicle trafficking. Related references: PMID:38538795
• Governs early-to-late endosomal maturation, microtubule-dependent migration and positioning, and endosome-lysosome transport.
• Plays a central role in growth-factor-mediated cell signaling, nutrient uptake, neurotrophin transport in axons, and lipid metabolism.
• Involved in specialized trafficking such as melanosome maturation, pathogen-induced phagosome processing, and autophagosome maturation.
• Controls endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Related references: PMID:11179213, PMID:12944476, PMID:14617358
• Required for exosomal release of SDCBP, CD63, and syndecan. Related references: PMID:22660413
• Required for vesicular trafficking and cell surface expression of ACE2. Related references: PMID:33147445
• Subcellularly localized to late endosome, lysosome, phagosome, autophagosome, melanosome, and lipid droplet membranes; widely expressed with high expression in skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 100-207 of human RAB7 (NP_004628.4). This region may provide target specificity; verify reactivity in your model system.
• For Western blotting, a band near the predicted molecular weight of 23 kDa is typically observed. Validate molecular weight markers and loading controls in your sample types.
• In immunoprecipitation, consider whether the antibody recognizes native conformations and optimize lysis conditions and antibody amounts.
• For ELISA, perform checkerboard titrations to determine optimal coating antigen and antibody concentrations; include relevant controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated IVD raw-material partner, offering streamlined access to validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For RAB7 and related endosomal trafficking and autophagy research, we provide comprehensive procurement support to accelerate your diagnostic development from concept to clinic.