Anti-RAB7 Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P51149

Core Product Specifications and Parameters

Biological Background: RAB7 Function and Localization

• RAB7A encodes Ras-related protein Rab-7a, a member of the Rab family of small GTPases that function as key regulators of intracellular membrane trafficking.
• RAB7A is a key regulator of endo-lysosomal trafficking, governing early-to-late endosomal maturation, microtubule-dependent endosomal positioning, and endosome-lysosome fusion. Related references: PMID:38538795
• RAB7A mediates growth factor signaling by controlling the endocytic trafficking and lysosomal degradation of receptor tyrosine kinases like EGFR and NTRK1/TRKA. Related references: PMID:11179213, PMID:12944476, PMID:14617358
• RAB7A participates in specialized endosomal pathways, including melanosome maturation, phagosome acidification against pathogens (e.g., S. aureus, M. tuberculosis), and autophagosome trafficking.
• RAB7A is essential for the exosomal release of SDCBP, CD63, and syndecan, and mediates the cell surface trafficking of ACE2, linking it to viral entry processes. Related references: PMID:22660413, PMID:33147445
• Subcellular localization includes late endosomes, lysosomes, phagosomes, autophagosomes, melanosomes, and mitochondria, reflecting its dynamic role in membrane trafficking.
• Tissue specificity: widely expressed, with particularly high expression in skeletal muscle.
• Associated keywords: Charcot-Marie-Tooth disease type 2B, autophagy, lipid metabolism, host-virus interaction, and protein transport, highlighting its roles in health and disease.

Experimental Guidance and Technical Tips

• The immunogen (a synthetic peptide from the C-terminal region, amino acids 108–207) is conserved in human, mouse, and rat RAB7, which likely contributes to the observed cross-reactivity. Verify sequence alignment for other species.
• For Western blotting, a band around 23 kDa is expected. Consider using reducing conditions and appropriate loading controls. Membrane protein enrichment may enhance detection.
• In immunofluorescence/immunocytochemistry (IF/ICC), anticipate endosomal and lysosomal staining; co-localization studies with organelle markers (e.g., EEA1 for early endosomes, LAMP1 for lysosomes) are recommended.
• For immunohistochemistry (IHC-P), test antigen retrieval methods (heat-induced or enzymatic) and validate with relevant positive and negative tissue controls.
• In ELISA, screen suggested antibody dilutions against recombinant RAB7 protein or cell lysates to determine optimal concentrations for capture or detection formats.
• Cross-adsorption against closely related RAB isoforms may improve specificity if required; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for IVD raw materials, technical services, and consulting, supporting manufacturers and labs from concept to clinic. For endo-lysosomal trafficking and neuropathology research, we offer validated monoclonal antibodies like this anti-RAB7 clone, along with optimized antibody pairs, bulk ancillary reagents, and rare target sourcing. Contact us to discuss your custom reagent needs and accelerate your diagnostic development.