Anti-RAB5C / RABL Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P51148

Core Product Specifications and Parameters

Biological Background: RAB5C / RABL Function and Localization

• Ras-related protein Rab-5C (RAB5C), also known as RABL or L1880, is a small GTPase of the Rab family that acts as a molecular switch in intracellular membrane trafficking.
• The protein cycles between an inactive GDP-bound and an active GTP-bound state, regulating transport vesicle formation, movement, tethering, and fusion with target membranes. Related references: PMID:16086013 PMID:17562788
• RAB5C is specifically involved in early endocytic trafficking and is required for the recruitment of the early endosomal autoantigen EEA1 to early endosomes. Related references: PMID:16086013 PMID:17562788
• It plays an essential role in the endocytosis of epidermal growth factor (EGF) and transferrin and their subsequent trafficking through early endosomes. Related references: PMID:16086013 PMID:17562788
• Subcellularly, RAB5C is localized to the cell membrane, early endosome membrane, and melanosome.
• Post-translational modifications include prenylation, phosphorylation, and glycosylation, which regulate its membrane association and activity.
• RAB5C contains conserved GTP-binding and magnesium-binding sites and exists in multiple isoforms due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly the full-length human RAB5C protein (residues 2–216), providing high specificity for the endogenous target.
• For western blotting, anticipate a band at approximately 23 kDa under standard reducing conditions; optimize blocking and antibody dilution in the relevant cell or tissue lysate.
• In immunofluorescence/ICC and immunoprecipitation assays, validate fixation, permeabilization, and lysis buffer conditions for your specific experimental system.
• The antibody exhibits reported cross-reactivity with mouse and rat orthologs; confirm suitability by testing in the appropriate species-derived samples.

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CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutions by offering one-stop access to validated IVD raw materials and technical services. Whether you need antibody pairs, bulk ancillary reagents, or assistance in sourcing rare targets for endocytic trafficking research, our platform supports you from concept to clinic. Explore how this anti-RAB5C monoclonal antibody and our broader portfolio can accelerate your diagnostic development.