Anti-RAB5B Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - P61020

Core Product Specifications and Parameters

Biological Background: RAB5B Function and Localization

• RAB5B encodes Ras-related protein Rab-5B, a member of the Rab family of small GTPases that serve as master regulators of intracellular membrane trafficking.
• The protein functions as a molecular switch by cycling between an inactive GDP-bound state and an active GTP-bound state, thereby recruiting specific effector proteins to mediate vesicle formation, movement, tethering, and fusion.
  Related references:
  PMID:16086013 | PMID:17562788
• RAB5B plays a critical role in early endocytic trafficking: it is required for the recruitment of early endosome antigen 1 (EEA1) to nascent endosomes, a key step in endosome maturation.
  Related references:
  PMID:16086013 | PMID:17562788
• It is essential for the endocytosis and proper trafficking of epidermal growth factor (EGF) and transferrin through the early endosomal compartment.
  Related references:
  PMID:16086013 | PMID:17562788
• Subcellularly, RAB5B localizes predominantly to the cytoplasmic face of early endosome membranes, but is also found at the cell membrane and in melanosomes.
• The protein undergoes post‑translational modifications including acetylation, phosphorylation, and prenylation, which modulate its activity, membrane anchoring, and interactions with regulatory proteins.
• Alternative splicing of the RAB5B gene produces multiple isoforms, and the protein is classified as a glycoprotein and lipoprotein based on its post‑translational processing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 100–200 of human RAB5B (NP_002859.1). This region is conserved across human, mouse, and rat, consistent with the observed multi‑species reactivity. Users should validate performance in their own sample systems.
• For Western blot, a specific band at ~24 kDa is expected under reducing conditions; consider including appropriate positive and negative controls (e.g., cell lines with known RAB5B expression) to confirm specificity.
• In immunofluorescence (IF/ICC) and immunofluorescence‑paraffin (IF‑P), RAB5B shows a punctate early endosomal pattern. Co‑staining with an EEA1 antibody or endosomal markers is recommended to verify localization.
• For ELISA, the antibody can be explored as a capture or detection reagent; however, optimal dilutions and pairing with other antibodies must be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a single‑point access to validated antibody pairs, monoclonals, polyclonals, and bulk ancillary reagents. This anti‑RAB5B polyclonal antibody supports research in intracellular trafficking, endocytosis, and membrane dynamics—areas critical for understanding disease mechanisms and developing diagnostic assays. From discovery to clinical application, our team streamlines your raw‑material procurement so you can focus on innovation.