Anti-RAB5A Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P20339

Core Product Specifications and Parameters

Biological Background: RAB5A Function and Localization

• RAB5A (Ras-related protein Rab-5A) is a small GTPase and a key regulator of intracellular membrane trafficking, cycling between inactive GDP-bound and active GTP-bound states to control vesicle formation, movement, and fusion. Related references: PMID:10818110, PMID:14617813, PMID:15378032
• It is essential for the fusion of plasma membranes with early endosomes and for early endocytic trafficking, including EGF and transferrin endocytosis. Related references: PMID:16086013, PMID:17562788
• RAB5A recruits EEA1 and RABEP1/Rabaptin-5 to early endosomes, promoting endocytic membrane fusion. Related references: PMID:16086013, PMID:17562788
• It contributes to filopodia extension and is required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Related references: PMID:14978216, PMID:22660413
• RAB5A localizes to the cell membrane, early endosome membrane, melanosomes, cytoplasmic vesicles, ruffles, and the phagosome membrane.
• It is involved in the recruitment of the FERRY complex to early endosomes, linking them with mRNAs for intracellular distribution. Related references: PMID:37267906
• Post-translational modifications include prenylation, phosphorylation, and glycosylation (by similarity); it binds GTP and magnesium, functioning as a hydrolase.
• RAB5A regulates maturation of apoptotic cell-containing phagosomes, likely downstream of DYN2 and PIK3C3 (by similarity).

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human RAB5A protein (aa 1-215), so the antibody may recognize both GDP- and GTP-bound conformations; consider this when interpreting localization and activity states.
• For Western blot (WB), the expected molecular weight is ~24 kDa; validate the band in your specific sample system and include appropriate controls.
• In IHC-P and IF/ICC, antigen retrieval and cell permeabilization steps are critical; optimize protocols for your target tissue or cell line.
• The antibody is predicted to cross-react with mouse and rat RAB5A, but sequence alignment and experimental validation in these species are recommended.
• For ELISA applications, consider using the recombinant immunogen or purified protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, supporting diagnostic manufacturers and researchers from early development to clinical application. This anti-RAB5A polyclonal antibody is an example of our portfolio; we also supply validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and can assist with sourcing rare-target raw materials for membrane trafficking and endocytosis research. Contact our team to streamline your procurement and accelerate your assay development.