Anti-RAB5A Monoclonal Antibody for WB, IHC-P, ELISA - P20339

Core Product Specifications and Parameters

Biological Background: RAB5A Function and Localization

• RAB5A belongs to the Rab family of small GTPases, which are master regulators of intracellular membrane trafficking, cycling between inactive GDP-bound and active GTP-bound states. Related references: PMID:10818110 PMID:14617813 PMID:15378032
• It plays a critical role in early endocytic trafficking by facilitating fusion of plasma membranes and early endosomes, and is essential for the recruitment of EEA1 to early endosomes. Related references: PMID:16086013 PMID:17562788
• RAB5A is required for efficient internalization and trafficking of EGF and transferrin through early endosomes. Related references: PMID:16086013 PMID:17562788
• The protein recruits the FERRY complex to early endosomes, linking endosomes with specific mRNAs to enable intracellular mRNA distribution. Related references: PMID:37267906
• RAB5A contributes to the regulation of filopodia extension. Related references: PMID:14978216
• It is necessary for the exosomal release of SDCBP, CD63, PDCD6IP, and syndecan. Related references: PMID:22660413
• The protein is localized to various membrane compartments including cell membrane, early endosome membrane, melanosomes, cytoplasmic vesicles, ruffles, phagosome membrane, and endosome membrane, reflecting its dynamic role in membrane trafficking.
• Post-translational modifications include prenylation (lipoprotein), phosphorylation (phosphoprotein), and glycosylation (glycoprotein), which are critical for its membrane association and function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 115–215 of human RAB5A; this region may be considered for functional domain studies or epitope mapping.
• For Western blot analysis, a specific band around 22–23 kDa is expected; ensure proper sample preparation and validate with appropriate positive controls (e.g., HeLa, HEK293 cell lysates).
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval may be required; consider testing dilutions and conditions on known positive tissues (e.g., liver, brain) that express RAB5A.
• For ELISA applications, the antibody can be utilized as a capture or detection reagent; performance should be validated in the relevant assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies and reagents for research and diagnostic development. This anti-RAB5A monoclonal antibody supports studies in endocytosis, membrane trafficking, and related pathways, and is available alongside customized antibody pairs, bulk ancillary reagents, and rare target sourcing solutions. Whatever your R&D demands—from single specificities to full-scale supply—CamelBio bridges the gap between concept and clinic.