Anti-RAB4A Monoclonal Antibody for WB - P20338

Core Product Specifications and Parameters

Biological Background: RAB4A Function and Localization

• RAB4A is a member of the Rab family of small GTPases, which are key regulators of intracellular membrane trafficking, including the formation, movement, tethering, and fusion of transport vesicles. Related references: PMID:15907487, PMID:16034420
• It cycles between an inactive GDP-bound form and an active GTP-bound form that recruits downstream effectors to membranes, orchestrating vesicular traffic.
• RAB4A specifically mediates VEGFR2 endosomal trafficking, enhancing VEGFR2 signaling and contributing to protein transport. Related references: PMID:29425100
• It also acts as a regulator of platelet alpha-granule release during platelet activation and aggregation (by similarity).
• Subcellularly, RAB4A localizes to the cytoplasm, and to membranes of early endosomes and recycling endosomes.
• Post-translational modifications and functional features include prenylation, phosphorylation, and GTP-binding, as reflected by its UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal peptide (amino acids 1-100); consider epitope accessibility when selecting applications.
• For Western blotting, a starting dilution range of 1:500–1:2000 is commonly used; optimization with positive control lysates from human or mouse cells is recommended.
• In ELISA, the antibody may be applied as a capture or detection reagent; validate under your specific plate-coating and blocking conditions.
• Due to the conserved nature of RAB4A, cross-reactivity with other species may occur; confirm specificity in your sample system.
• The predicted band size is approximately 24 kDa, but post-translational modifications may result in slight mobility shifts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For studies of intracellular trafficking, endosomal dynamics, and VEGFR2 signaling, our RAB4A monoclonal antibody offers a reliable tool for WB and ELISA applications. Leverage CamelBio’s integrated supply chain to secure rare-target raw materials and support your immunoassay development from concept to clinic.