Anti-RAB45 Rabbit Monoclonal Antibody for WB and ELISA - Q8IZ41

Core Product Specifications and Parameters

Biological Background: Ras and EF-hand domain-containing protein Function and Localization

• RASEF (RAB45) encodes a Rab GTPase belonging to the RAS superfamily, characterized by a unique combination of Ras and EF-hand domains, and undergoes alternative splicing to generate multiple isoforms.
• The primary function of RASEF is to act as a dynein adapter protein, activating dynein-mediated transport and dynein-dynactin motility on microtubules in a Ca(2+)-independent manner. Related references: PMID:30814157
• RASEF also regulates the formation and sorting of zymogen granules and secretion of digestive enzymes by pancreatic acinar cells, contributing to exocrine pancreas function.
• Subcellularly, RASEF localizes to the perinuclear cytoplasm, Golgi apparatus, early and late endosomes, and lysosomes, indicating roles in intracellular vesicle trafficking.
• The protein contains calcium-binding EF-hand motifs and GTP-binding domains, and is regulated by nucleotide binding and phosphorylation, as indicated by its keywords.
• Tissue specificity analysis shows RASEF is down-regulated in cutaneous melanoma cells, suggesting a potential role in melanocyte biology or tumor suppression.

Experimental Guidance and Technical Tips

• The immunogen consists of the N-terminal 120 amino acids (mapping to the Ras-like domain), which may enable recognition of conserved epitopes across human and mouse species due to high sequence homology.
• For Western blotting, the predicted molecular weight is ~83 kDa; consider using whole-cell lysates from human or mouse cell lines (e.g., HeLa, NIH/3T3) or pancreatic tissue, and validate with appropriate loading controls.
• In ELISA applications, the antibody may be used for capturing or detecting recombinant or endogenous RAB45; consider optimizing coating conditions and verifying specificity using recombinant protein standards.
• As cross-reactivity is reported for human and mouse, this antibody can facilitate translational studies; nonetheless, always confirm reactivity in the relevant sample system before quantitative assays.
• Given the protein's dynein adapter function, researchers might explore its utility in co-immunoprecipitation or proximity ligation assays to study protein interactions, though these have not been validated for this product.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we understand the critical need for reliable, high-quality raw materials in diagnostic development. This anti-RASEF (RAB45) monoclonal antibody, suited for WB and ELISA, exemplifies our commitment to providing validated, ready-to-use reagents for intracellular trafficking and cancer research. Beyond individual antibodies, CamelBio offers comprehensive sourcing services including matched antibody pairs, bulk reagent supply, and custom raw material development to streamline your IVD assay development from concept to clinic.