Anti-RAB3GAP1 Polyclonal Antibody for WB, ELISA - Q15042

Core Product Specifications and Parameters

Biological Background: RAB3GAP1 (Rab3 GTPase-activating protein catalytic subunit) Function and Localization

• RAB3GAP1 encodes the catalytic 130 kDa subunit of the Rab3 GTPase-activating protein complex, also known as p130, and is associated with Warburg Micro syndrome (WARBM1) and Martsolf syndrome.
• It partners with the non-catalytic subunit RAB3GAP2 to form the functional Rab3GAP complex, which accelerates the slow intrinsic GTP hydrolysis by Rab proteins, including Rab3 subfamily members (RAB3A-D), RAB5A, and RAB43. Related references: PMID:9030515 PMID:10859313 PMID:39779760
• Beyond its GAP activity, RAB3GAP1 exhibits guanine nucleotide exchange factor (GEF) activity toward RAB18, promoting the conversion of inactive RAB18-GDP to active RAB18-GTP. Related references: PMID:24891604 PMID:39779760
• The protein localizes to the cytoplasm, endoplasmic reticulum (ER), and Golgi apparatus, where it recruits and stabilizes RAB18 at the cis-Golgi and ER membranes to maintain proper organelle structure. Related references: PMID:26063829 PMID:24891604
• RAB3GAP1 is essential for normal eye and brain development; mutations lead to developmental defects and neurological disorders. Related references: PMID:15696165 PMID:23420520
• It may participate in early neurodevelopmental processes such as cell proliferation, migration, and differentiation, and in regulating non-synaptic vesicular release of neurotransmitters. Related references: PMID:9030515 PMID:9852129
• The protein is ubiquitously expressed across tissues, with known phosphorylation sites and multiple splice isoforms, and is catalogued under keywords including cataract, intellectual disability, and disease variant.

Experimental Guidance and Technical Tips

• For Western blotting, a typical starting dilution range is 1:500–1:2000; titrate to optimize signal-to-noise for your specific sample type and expression level.
• In ELISA, consider using the recombinant immunogen (aa 500-780) as a positive control to confirm detection of the target antigen.
• As a polyclonal antibody, it may recognize multiple epitopes; validate specificity by comparing reactivity in knockout versus wild-type lysates or by siRNA knockdown.
• Given the immunogen region, note potential cross-reactivity with splice variants or closely related proteins; confirm band identity with molecular weight markers (expected ~111 kDa).
• For species predicted to react (human, mouse, rat), verify reactivity in your experimental system due to possible sequence divergence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a seamless supply of IVD raw materials, spanning validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. For RAB3GAP1-related research in neurodevelopment, intracellular trafficking, and membrane dynamics, our product portfolio ensures reliable access to high-quality reagents, enabling your projects from concept to clinical application.