Anti-RAB38 Polyclonal Antibody for WB, ELISA - P57729

Core Product Specifications and Parameters

Biological Background: RAB38 Function and Localization

• Ras-related protein Rab-38, also known as Melanoma antigen NY-MEL-1, belongs to the small GTPase Rab family.
• Small GTPases of the Rab family are key regulators of intracellular membrane trafficking, cycling between an inactive GDP-bound form and an active GTP-bound form that recruits downstream effectors responsible for vesicle formation, movement, tethering, and fusion.
• RAB38 is involved in melanosomal transport and docking, contributing to the proper sorting and peripheral melanosomal distribution of TYRP1 in melanocytes, in cooperation with ANKRD27 and VAMP7.
• A role in phagosome maturation has been reported; RAB38 participates in the maturation of phagosomes that engulf pathogens such as S.aureus and M.tuberculosis. Related references: PMID:21255211
• RAB38 is important for the control of melanin production and melanosome biogenesis, and together with RAB32, regulates the trafficking of melanogenic enzymes TYR, TYRP1, and DCT/TYRP2 to melanosomes. Related references: PMID:23084991
• Subcellular localization includes the cell membrane, melanosomes, cytoplasmic vesicles, and phagosome membranes.
• Predominantly expressed in melanocytes.
• Possesses conserved GTP-binding and hydrolysis domains and undergoes post-translational modifications such as palmitoylation and prenylation, typical of Rab GTPases.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 92–211 of human RAB38, a region conserved across human, mouse, and rat, likely contributing to the reported cross-reactivity.
• For Western blot, the predicted molecular weight is approximately 24 kDa; consider validating with positive control lysates from melanocyte or melanoma cell lines.
• For ELISA, test with recombinant RAB38 protein or cell lysates, and optimize coating and detection conditions for your specific experimental system.
• Since RAB38 expression is tissue-specific, use appropriate biological samples and consider enrichment strategies for low-abundance targets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutions with streamlined access to validated IVD raw materials, including antibodies targeting intracellular trafficking and melanocyte biology. From optimized polyclonal antibodies like anti-RAB38 to ancillary reagents and rare-target sourcing, CamelBio supports your development workflow from concept to clinic. Let us be your procurement partner for building robust melanosome research and phagosome maturation assays.