Anti-RAB33A (RabS10) Rabbit Polyclonal Antibody for WB and ELISA - Q14088

Core Product Specifications and Parameters

Biological Background: RAB33A Function and Localization

• RAB33A (Ras-related protein Rab-33A, also known as RabS10) is a small GTPase belonging to the Rab protein family, which are key regulators of intracellular membrane trafficking.
• Rab proteins control the entire lifecycle of transport vesicles, including formation, movement, tethering, and fusion, through cycling between inactive GDP-bound and active GTP-bound states.
• RAB33A specifically modulates autophagosome formation by interacting with ATG16L1, a core component of the autophagy machinery.
• The protein is localized to the cell membrane, consistent with its role in membrane trafficking events.
• RAB33A is expressed exclusively in lymphoid cell lines, suggesting a specialized function in the immune system.
• Post-translational modifications such as prenylation, methylation, and lipidation are critical for its membrane association and functional regulation.
• The predicted molecular weight is approximately 26.6 kDa, but it migrates around 27 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fragment corresponding to the C-terminal region (amino acids 118–237), which may generate antibodies recognizing endogenous RAB33A in denaturing Western blot and native ELISA formats.
• As a polyclonal antibody, it can detect multiple epitopes, potentially enhancing sensitivity but requiring careful validation for specificity.
• For Western blot analysis, consider testing a range of dilutions (e.g., 1:500 – 1:2000) and use lysates from lymphoid cell lines where RAB33A is endogenously expressed.
• Cross-reactivity with mouse and rat RAB33A has been reported, likely due to high sequence homology; validate in the relevant species’ tissues or cell lines.
• Given the protein’s roles in autophagy and vesicle trafficking, functional studies may benefit from complementary approaches such as immunofluorescence or co-immunoprecipitation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. For RAB33A and related autophagy or trafficking targets, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing of rare-target raw materials. Contact us to streamline your procurement and accelerate your diagnostic development with reliable, high-quality reagents.