Anti-RAB31 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13636

Core Product Specifications and Parameters

Biological Background: RAB31 Function and Localization

• RAB31 encodes Ras-related protein Rab-31 (also known as Rab-22B), a small GTPase belonging to the Rab family of key regulators of intracellular membrane trafficking. Related references: PMID:11784320
• RAB31 cycles between an inactive GDP-bound form and an active GTP-bound form, recruiting downstream effectors that drive vesicle formation, transport, tethering, and fusion.
• It is required for the structural integrity and normal function of the Golgi apparatus and the trans-Golgi network (TGN), and participates in insulin-stimulated GLUT4 translocation to the plasma membrane.
• RAB31 mediates mannose-6-phosphate receptor (M6PR) trafficking from the TGN to endosomes, and promotes internalization of the epidermal growth factor receptor (EGFR) into endosomes.
• In immune cells, RAB31 contributes to phagosome maturation against engulfed pathogens such as Staphylococcus aureus and Mycobacterium tuberculosis.
• Subcellular localization includes Golgi apparatus, trans-Golgi network membrane, early endosomes, and phagosome membranes, reflecting its dynamic membrane-cycling behavior.
• Highest expression is observed in placenta and brain, with lower levels in heart and lung; it is undetectable in liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include phosphorylation and prenylation (lipoprotein) that likely regulate membrane association; the protein is identified in proteomics datasets.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length recombinant protein (aa 1–195), which may recognize both GDP- and GTP-bound conformations; verify binding specificity under different nucleotide conditions if required.
• For Western blot (WB), consider loading appropriate positive controls from human, mouse, or rat tissue/cell lysates known to express RAB31 (e.g., brain, placenta). A band near 22 kDa is expected.
• In immunofluorescence/ICC (IF/ICC), because RAB31 localizes to dynamic membranous compartments, use gentle fixation (e.g., 4% PFA) and co-stain with organelle markers (TGN, early endosomes) to confirm signal specificity.
• For ELISA, titrate both antibody and antigen to determine optimal working concentrations; use a recombinant RAB31 protein or peptide as coating antigen.
• Because the antibody is polyclonal, lot-to-lot consistency should be validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. As a one-stop sourcing partner, we supply validated polyclonal and monoclonal antibodies (including target pairs), bulk ancillary reagents, and assist in locating rare-target raw materials for membrane trafficking studies. Whether you are developing assays around RAB31-dependent pathways or scaling up production, CamelBio bridges your procurement needs with dependable supply solutions.