Anti-RAB31 Polyclonal Antibody for WB, ELISA - Q13636

Core Product Specifications and Parameters

Biological Background: RAB31 Function and Localization

• RAB31, also known as Rab-22B, is a member of the Ras-related Rab subfamily of small GTPases involved in intracellular membrane trafficking regulation. Related references: PMID:11784320
• It cycles between an inactive GDP-bound form and an active GTP-bound form, recruiting effectors for vesicle formation, movement, tethering, and fusion.
• RAB31 is essential for Golgi apparatus integrity and normal function, particularly at the trans-Golgi network (TGN).
• It participates in insulin-stimulated translocation of GLUT4 to the plasma membrane, playing a role in glucose transport.
• RAB31 mediates transport of mannose-6-phosphate receptor (M6PR) from the TGN to endosomes, and facilitates internalization of EGFR from the cell surface into endosomes.
• The protein also contributes to phagosome maturation, aiding in the defense against pathogens such as S. aureus and M. tuberculosis.
• Subcellularly, RAB31 localizes to the Golgi apparatus, trans-Golgi network membrane, early endosomes, and phagosome membranes.
• Highest tissue expression is observed in placenta and brain, with lower levels in heart and lung; it is undetectable in liver, skeletal muscle, kidney, or pancreas.

Experimental Guidance and Technical Tips

• The immunogen spans nearly full-length RAB31 (aa 1-195), which may provide robust detection of endogenous RAB31 across various species.
• For western blot, a band at ~22 kDa is expected; consider optimizing blocking and antibody dilution for your specific sample type.
• ELISA applications may benefit from using the recombinant immunogen as a positive control or coating antigen.
• Given cross-reactivity with mouse and rat, the antibody can be used to study RAB31 in rodent models, though validation in relevant tissue lysates is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RAB31 polyclonal antibody supports investigations into membrane trafficking, endocytosis, and Golgi biology. Our sourcing network can supply validated antibody pairs, bulk polyclonal/monoclonal antibodies, and ancillary reagents to streamline your IVD assay development for RAB31 and related targets.