Anti-RAB27B Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - O00194

Core Product Specifications and Parameters

Biological Background: RAB27B Function and Localization

• RAB27B, also known as C25KG, is a member of the Rab family of small GTPases.
• RAB27B functions as a key regulator of intracellular membrane trafficking, cycling between inactive GDP-bound and active GTP-bound states to recruit downstream effectors that orchestrate vesicle formation, movement, tethering, and fusion (PubMed:21775604, PubMed:30771381). Related references: PMID:21775604 PMID:30771381
• It specifically regulates homeostasis of the late endocytic pathway, controlling endosomal positioning, maturation, and secretion (PubMed:30771381). Related references: PMID:30771381
• RAB27B facilitates NTRK2/TRKB axonal anterograde transport by promoting the association of NTRK2/TRKB with KLC1 (PubMed:21775604). Related references: PMID:21775604
• The protein is localized to membranes and late endosomes.
• It is predominantly expressed in testis.
• Additional keywords indicate involvement in GTP-binding, hydrolase activity, and prenylation.

Experimental Guidance and Technical Tips

• Given the immunogen design spanning amino acids 1-218, this antibody may recognize epitopes within the N-terminal domain; consider validation in your specific experimental system.
• For Western blotting, a band at approximately 25kDa is expected; use appropriate positive controls such as testis lysate where expression is high.
• In immunoprecipitation and immunofluorescence protocols, ensure proper controls for membrane trafficking studies.
• The antibody's cross-reactivity with mouse and rat allows for use in rodent models; validate specificity in the relevant tissue or cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting development from concept to clinic. For researchers studying intracellular trafficking and endosomal regulation, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets like RAB27B. Partner with CamelBio to streamline your procurement of high-quality reagents and accelerate your diagnostic research.