Anti-RAB27B Polyclonal Antibody for WB, ELISA - O00194

Core Product Specifications and Parameters

Biological Background: RAB27B Function and Localization

• RAB27B is a Rab GTPase that acts as a molecular switch in intracellular membrane trafficking, cycling between inactive GDP- and active GTP-bound forms to regulate vesicle formation, movement, tethering, and fusion. Related references: PMID:21775604, PMID:30771381
• It specifically controls homeostasis of the late endocytic pathway, including endosome positioning, maturation, and secretion. Related references: PMID:30771381
• RAB27B facilitates anterograde axonal transport of the TrkB neurotrophin receptor (NTRK2) by coupling it to kinesin light chain KLC1. Related references: PMID:21775604
• May be involved in targeting uroplakins to the urothelial apical membrane (by similarity).
• Predominantly expressed in testis, suggesting specialized reproductive functions.
• Localizes to late endosomes and plasma membrane, consistent with its regulatory role in endosomal traffic.
• Annotated with post‑translational modifications including prenylation, acetylation, and methylation, which likely influence membrane anchoring and activity.

Experimental Guidance and Technical Tips

• The immunogen comprises the full‑length human RAB27B sequence (aa 1‑218); thus, the antibody is expected to recognize the native protein. Validate detection of the predicted 25 kDa band and any modified forms in the relevant sample system (e.g., testis lysate) before routine use.
• For Western blot, consider optimizing blocking and wash conditions to reduce non‑specific binding when using polyclonal sera. Perform a titration series to determine the optimal dilution.
• In ELISA, confirm appropriate coating and detection conditions; this antibody may be employed as a capture or detection reagent in sandwich formats if paired with a compatible antibody.
• Cross‑reactivity with mouse RAB27B is reported; however, sequence alignments should be checked if extending to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials spanning the entire development pipeline. Need validated polyclonal antibodies like this anti‑RAB27B reagent for endosomal trafficking studies or axonal transport research? We can supply optimized antibody pairs, bulk ancillary reagents, and rare‑target raw materials to accelerate your assay development and scale‑up. Contact us to discuss your specific sourcing requirements.