Anti-RAB27A Polyclonal Antibody (KD Validated) for WB, IF/ICC, ELISA - P51159

Core Product Specifications and Parameters

Biological Background: RAB27A Function and Localization

• RAB27A, also known as Ras-related protein Rab-27A or GTP-binding protein Ram, is a member of the Rab small GTPase family that regulates intracellular membrane trafficking.
• It cycles between an inactive GDP-bound and an active GTP-bound form, recruiting downstream effectors for vesicle formation, movement, tethering, and fusion (PubMed:30771381).
  Related references: PMID:30771381
• RAB27A plays a critical role in the homeostasis of the late endocytic pathway, including endosomal positioning, maturation, and secretion (PubMed:30771381).
  Related references: PMID:30771381
• It is essential for cytotoxic granule exocytosis in lymphocytes, regulating granule maturation, docking, and priming at the immunologic synapse (PubMed:18812475).
  Related references: PMID:18812475
• Subcellularly, RAB27A localizes to membranes of melanosomes, late endosomes, and lysosomes.
• It is widely expressed except in brain, with low levels in thymus, kidney, muscle, and placenta, and high expression in melanocytes, cytotoxic T-lymphocytes (CTLs), and mast cells.
• Post-translational modifications include prenylation (for membrane anchoring), acetylation, methylation, and phosphorylation, which influence its activity and localization.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length RAB27A (amino acids 1-221), so the polyclonal antibody may recognize multiple epitopes; consider validating specificity with knockdown or knockout controls.
• KD-validated status confirms target specificity in human cells, but cross-reactivity with mouse RAB27A should be confirmed individually.
• Recommended for WB, IF/ICC, and ELISA; optimize dilutions and blocking conditions according to your sample and assay setup.
• Shipment on ice bags ensures stability; store at recommended temperatures upon receipt to maintain performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, monoclonals, polyclonals, and ancillary reagents to support your intracellular trafficking and exocytosis research. Whether you need bulk quantities or assistance in sourcing rare targets like RAB27A, our team provides end-to-end solutions from discovery to clinical application. Connect with us to streamline your IVD development.