Anti-RAB22A Rabbit Monoclonal Antibody for WB, IP, ELISA - Q9UL26

Core Product Specifications and Parameters

Biological Background: RAB22A Function and Localization

• RAB22A, also known as ras-related protein Rab-22A, is a 22 kDa member of the Rab small GTPase family.
• As a key regulator of intracellular membrane trafficking, RAB22A cycles between inactive GDP-bound and active GTP-bound states, recruiting effectors for vesicle formation, movement, tethering, and fusion. Related references: PMID:16537905, PMID:21849477
• It specifically mediates the transport of transferrin/TF from early endosomes to recycling endosomes. Related references: PMID:16537905
• RAB22A is essential for NGF-dependent endocytosis of the NTRK1 receptor, a process critical for neurite outgrowth. Related references: PMID:21849477
• It displays low intrinsic GTPase activity, contributing to its regulatory dynamics. Related references: PMID:16537905
• The protein localizes to endosome membranes, early and late endosomes, the plasma membrane, cell ruffles, and phagosome membranes.
• RAB22A undergoes prenylation for membrane attachment, binds GTP and magnesium, and participates in endocytosis and protein transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 100 amino acids of human RAB22A; for WB, the predicted band is around 22 kDa—consider using 12-15% SDS-PAGE gels for optimal resolution.
• This antibody detects human, mouse, and rat RAB22A; validate specificity in the relevant species and tissue context using appropriate positive and negative controls.
• For IP, titrate the antibody amount and include an isotype control to minimize non-specific binding.
• For ELISA, follow standard direct or indirect protocols and confirm binding with recombinant RAB22A protein if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-RAB22A antibody supports research into endocytic trafficking and neurite outgrowth, and we can assist with sourcing rare target raw materials to accelerate your diagnostic development from concept to clinic.