Anti-RAB21 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UL25

Core Product Specifications and Parameters

Biological Background: RAB21 Function and Localization

• Ras-related protein Rab-21 (RAB21) is a member of the Rab family of small GTPases, which function as key regulators of intracellular membrane trafficking, including vesicle formation, movement, tethering, and fusion. RAB21 cycles between an inactive GDP-bound and an active GTP-bound state to recruit downstream effectors. Related references: PMID:18804435 PMID:25648148 PMID:31455601
• During mitosis of adherent cells, RAB21 controls the endosomal trafficking of integrins, a process essential for the successful completion of cytokinesis. Related references: PMID:18804435
• RAB21 regulates integrin internalization and recycling, thereby influencing cell adhesion and migration, without affecting the general traffic of endosomally translocated receptors.
• Following SBF2/MTMR13-mediated activation in response to starvation-induced autophagy, RAB21 binds to and regulates the SNARE protein VAMP8 to facilitate endolysosomal transport required for autophagosome-lysosome fusion. Related references: PMID:25648148
• RAB21 modulates protein levels of cargo receptors TMED2 and TMED10, and is required for proper Golgi localization of TMED10. Related references: PMID:31455601
• RAB21 is involved in neurite growth and is localized to various membrane compartments including the endoplasmic reticulum, Golgi apparatus, early endosomes, cytoplasmic vesicles, cleavage furrow, and neuron projections.
• Widely expressed across tissues, with prominent expression in the apical region of jejunal epithelial cells, capillary endothelium, and certain lamina propria cells.
• Post-translational modifications include acetylation and prenylation; the protein has a molecular weight of approximately 24 kDa (calculated 24348 Da).

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against a full-length recombinant RAB21 protein (aa 1-225), may detect endogenous RAB21 in a variety of species, including human, mouse, and rat, based on cross-reactivity data.
• For Western blotting, a band at approximately 24 kDa is expected; consider using appropriate loading controls and validating the antibody's specificity in your sample type.
• Immunofluorescence/ICC applications can be explored to visualize RAB21 localization at membranes and vesicles; optimize fixation and permeabilization conditions to preserve antigenicity.
• In ELISA, the antibody can be used for detection or capture; titrate the antibody to determine optimal working concentrations for your assay.
• Note that polyclonal antibodies exhibit lot-to-lot variability; for critical quantitative studies, we recommend confirming performance with each new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. For researchers studying intracellular trafficking and membrane dynamics, this RAB21 antibody serves as a reliable tool, and CamelBio can support your complete workflow—from bulk reagent procurement to rare target sourcing—helping you accelerate development from concept to clinic.