Anti-RAB20 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NX57

Core Product Specifications and Parameters

Biological Background: RAB20 Function and Localization

• RAB20 is a member of the Rab family of small GTPases, which are key regulators of intracellular membrane trafficking, cycling between an inactive GDP-bound and an active GTP-bound state.
• It is involved in apical endocytosis and recycling pathways, contributing to the dynamic organization of endomembrane compartments.
• RAB20 specifically functions in the maturation and acidification of phagosomes during engulfment of pathogens like Staphylococcus aureus and Mycobacterium tuberculosis.
• It also plays a critical role in mediating the fusion of phagosomes with lysosomes, a key step in pathogen clearance.
• Subcellularly, RAB20 localizes to the Golgi apparatus, cytoplasmic vesicles, and the phagosome membrane, consistent with its trafficking roles.
• Tissue expression is generally low in normal pancreas but is strongly upregulated in exocrine pancreatic adenocarcinomas (at protein level), suggesting a role in cancer.
• As a GTP-binding protein, RAB20 requires magnesium for nucleotide binding and is post-translationally prenylated for membrane attachment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80–190 of human RAB20, a central region likely spanning the GTPase domain; antibody reactivity may be influenced by the activation state or prenylation status of the target.
• For Western blot, a band near 26 kDa is expected; consider using appropriate lysis conditions and positive controls (e.g., pancreatic adenocarcinoma cell lines) to confirm specificity.
• In IF/ICC, validate staining in cells with known RAB20 expression, and be aware that localization may shift between Golgi, vesicles, and phagosomes depending on cellular context.
• Cross-reactivity with mouse is indicated; when using in mouse models, verify reactivity in the relevant tissue or cell type.
• For ELISA, optimize coating antigen and detection conditions; the antibody may recognize both recombinant and native RAB20.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive, one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for RAB20 and related Rab family proteins. Whether you are developing assays for intracellular trafficking, phagocytosis, or pancreatic cancer markers, CamelBio streamlines procurement from concept to clinic with reliable supply chains and technical consulting.