Anti-RAB1B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H0U4

Core Product Specifications and Parameters

Biological Background: RAB1B Function and Localization

• RAB1B is a member of the Rab family of small GTPases, acting as a key regulator of intracellular membrane trafficking from the formation of transport vesicles to their fusion with target membranes. It cycles between an inactive GDP-bound form and an active GTP-bound form that recruits downstream effectors responsible for vesicle formation, movement, tethering, and fusion. Related references: PMID:20545908 PMID:9437002 PMID:23236136

• Plays a role in the early stages of autophagic vacuole development, which occurs at specialized endoplasmic reticulum subdomains. Related references: PMID:20545908

• Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.

• Required for maintaining the compacted morphology of the Golgi apparatus. Related references: PMID:26209634

• Promotes the recruitment of the lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment.

• Subcellularly localized to the cytoplasm, membrane, preautophagosomal structure membrane, and perinuclear region.

• Post-translationally modified by acetylation, methylation, phosphorylation, prenylation, and glycosylation, which modulate its activity and membrane association.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 7-201 of human RAB1B, corresponding to a large portion of the protein; the polyclonal antibody may target multiple epitopes, making it well-suited for applications like WB and IF/ICC.
• For Western blotting, expect a band at approximately 22 kDa; optimize sample preparation and loading controls.
• For immunofluorescence/immunocytochemistry, appropriate fixation and permeabilization protocols should be optimized for membrane and cytosolic proteins.
• ELISA can be employed for antibody titration and specificity testing; include relevant controls.
• Validate reactivity in your specific sample system (e.g., human, mouse, rat) as cross-reactivity has been reported; note that endogenous expression levels may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development. This anti-RAB1B antibody supports research into intracellular trafficking and autophagy pathways. Contact us to discuss customized supply solutions tailored to your project needs.