Anti-RAB1B Rabbit Polyclonal Antibody for WB, ELISA - Q9H0U4

Core Product Specifications and Parameters

Biological Background: RAB1B Function and Localization

• RAB1B is a member of the Rab family of small GTPases, acting as a molecular switch in intracellular membrane trafficking pathways.
• It cycles between an inactive GDP-bound state and an active GTP-bound state, recruiting downstream effectors responsible for vesicle formation, movement, tethering, and fusion. Related references: PMID:9437002
• RAB1B plays a critical role in the initial events of autophagic vacuole development, which occurs at specialized regions of the endoplasmic reticulum. Related references: PMID:20545908
• In the secretory pathway, RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
• It is required to maintain the compacted morphology of the Golgi apparatus. Related references: PMID:26209634
• RAB1B promotes the recruitment of the lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment.
• Subcellularly, RAB1B localizes to the cytoplasm, membrane, preautophagosomal structure membrane, and perinuclear region.
• Post-translational modifications include acetylation, phosphorylation, and prenylation, which are critical for its membrane association and function.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 7–201 of human RAB1B, a region that is well-conserved; predicted cross-reactivity with mouse should be verified experimentally in the relevant sample system.
• For Western blotting, consider a primary antibody dilution range of 1:500–1:2000; optimize detection conditions using cell lysates known to express RAB1B (molecular weight ~22 kDa).
• This antibody is suitable for ELISA applications; to achieve optimal sensitivity, validate coating concentrations and detection parameters with your specific assay format.
• Given the role of RAB1B in autophagy and ER–Golgi trafficking, consider using appropriate controls and co-staining to confirm localization in functional studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to a comprehensive portfolio of IVD raw materials. For RAB1B research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing of rare-target raw materials. Our integrated supply chain supports projects from early feasibility to commercial scale, enabling you to accelerate your intracellular trafficking and autophagy studies.