Anti-RAB1A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62820

Core Product Specifications and Parameters

Biological Background: RAB1A Function and Localization

• RAB1A is a small GTPase of the Rab family that cycles between an inactive GDP-bound form and an active GTP-bound form, serving as a key regulator of intracellular membrane trafficking from the formation of transport vesicles to their fusion with membranes. Related references: PMID:20639577, PMID:20861236, PMID:21303926
• It specifically regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and onwards to the cell surface, and is involved in IL-8 and growth hormone secretion. Related references: PMID:21303926
• RAB1A is required to modulate the compacted morphology of the Golgi apparatus. Related references: PMID:26209634
• It regulates the level of the calcium-sensing receptor (CASR) present at the cell membrane. Related references: PMID:20861236
• RAB1A plays a role in cell adhesion and cell migration via its function in protein trafficking. Related references: PMID:20639577
• It is involved in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Related references: PMID:22939626
• Subcellularly, RAB1A localizes to the Golgi apparatus, endoplasmic reticulum, early endosome, cytosol, membrane, and melanosomes.
• The protein undergoes various post-translational modifications including prenylation, acetylation, phosphorylation, and ubiquitination-like modifications, and exists in alternatively spliced isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human RAB1A, potentially recognizing both GDP- and GTP-bound forms. Consider relevant controls for activation state-specific detection.
• For Western blot, a band near 23 kDa is expected; post-translational modifications may cause slight shifts. Validate in the relevant sample system using recommended loading controls.
• In immunofluorescence/immunocytochemistry, expect staining of the Golgi apparatus and endoplasmic reticulum; co-localization with organelle markers can confirm specificity.
• Indirect ELISA may be used for quantification; optimize antibody dilution and consider antigen coating conditions for best performance.
• The antibody cross-reacts with mouse and rat, making it suitable for studies in these model organisms; verify cross-reactivity in your specific sample context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-RAB1A antibody supports research in membrane trafficking, autophagy, and cell signaling, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your IVD development. Our expertise ensures seamless procurement and technical support tailored to your RAB1A-related projects.