Anti-RAB1A Polyclonal Antibody for WB, IF/ICC, ELISA - P62820

Core Product Specifications and Parameters

Biological Background: RAB1A Function and Localization

• RAB1A, also known as Ras-related protein Rab-1A or YPT1-related protein, is a small GTPase encoded by the RAB1A gene in humans.
• It functions as a key regulator of intracellular membrane trafficking, cycling between inactive GDP-bound and active GTP-bound states that recruit distinct effector proteins to drive vesicle formation, movement, tethering, and fusion.
  Related references:
  PMID:20639577 PMID:20861236 PMID:21303926
• RAB1A specifically mediates vesicular protein transport from the endoplasmic reticulum to the Golgi apparatus and onward to the cell surface, and is required for secretion of IL-8 and growth hormone.
  Related references:
  PMID:21303926
• It plays a role in maintaining the compacted morphology of the Golgi apparatus.
  Related references:
  PMID:26209634
• RAB1A regulates the cell-surface abundance of the calcium-sensing receptor (CASR).
  Related references:
  PMID:20861236
• Through its trafficking functions, RAB1A contributes to cell adhesion and cell migration.
  Related references:
  PMID:20639577
• It is involved in autophagosome assembly and cellular defense responses against pathogenic bacteria.
  Related references:
  PMID:22939626
• Subcellular localization includes the Golgi apparatus, endoplasmic reticulum, early endosomes, cytosol, membrane, and melanosomes. Post-translational modifications such as acetylation, prenylation, and phosphorylation have been reported.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 23 kDa is expected; validate with appropriate positive and negative controls, and consider using loading controls to confirm equal protein loading.
• In immunofluorescence and immunocytochemistry, optimize fixation and permeabilization conditions for the specific cell type and subcellular compartments of interest (e.g., Golgi/ER).
• When using in ELISA, appropriate antigen coating and antibody titration are recommended; the antibody may be suitable as a capture or detection reagent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials for diagnostic manufacturers and researchers. This RAB1A polyclonal antibody aids in studying ER-Golgi trafficking, autophagy, and related pathways. We offer validated antibody pairs, optimized antibodies, bulk ancillary reagents, and rare-target sourcing with dedicated technical consultation throughout the development pipeline.