Anti-RAB18 Polyclonal Antibody for WB and ELISA - Q9NP72

Core Product Specifications and Parameters

Biological Background: RAB18 Function and Localization

• RAB18 is a member of the Ras-related Rab family of small GTPases, key regulators of intracellular membrane trafficking, cycling between inactive GDP-bound and active GTP-bound forms. Related references: PMID:24891604, PMID:30970241
• It is required for the localization of ZFYVE1 to lipid droplets and for the formation of endoplasmic reticulum-lipid droplet (ER-LD) contact sites. Related references: PMID:30970241
• RAB18 is also essential for maintaining endoplasmic reticulum structure. Related references: PMID:24891604
• It plays a key role in eye and brain development and neurodegeneration. Related references: PMID:21473985
• Subcellularly, RAB18 localizes to the endoplasmic reticulum membrane, Golgi apparatus, cis-Golgi network membrane, lipid droplets, and apical cell membrane.
• The protein undergoes various post-translational modifications such as acetylation, methylation, phosphorylation, palmitoylation, and prenylation, and is involved in processes like protein transport and host-virus interactions.
• Ubiquitously expressed in human tissues.

Experimental Guidance and Technical Tips

• For western blotting, a band corresponding to the predicted molecular weight of RAB18 (approximately 23 kDa) is expected; consider using a suitable protein ladder and confirm specificity with appropriate controls.
• RAB18 is a membrane-associated protein; ensure efficient protein extraction by using lysis buffers containing detergents (e.g., 1% Triton X-100 or NP-40) to solubilize membrane fractions.
• The immunogen spans the full-length human RAB18 protein; cross-reactivity with mouse and rat has been reported, but validation in the specific sample system is recommended.
• For ELISA applications, use the recombinant protein as a positive control; optimize coating conditions and antibody dilutions for best signal-to-noise ratio.
• As a polyclonal antibody, it may recognize multiple epitopes; for critical experiments, consider knockdown or knockout validation to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide reliable, high-quality IVD raw materials. This RAB18 polyclonal antibody supports research into membrane trafficking, lipid droplet biology, and neurodegeneration. Beyond antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your assay development from concept to clinic.