Anti-RAB14 Rabbit Polyclonal Antibody for WB and ELISA - P61106

Core Product Specifications and Parameters

Biological Background: RAB14 Function and Localization

• RAB14 is a member of the Rab family of small GTPases, which function as key regulators of intracellular membrane trafficking from vesicle formation to membrane fusion.
• The protein cycles between an inactive GDP-bound form and an active GTP-bound form that recruits downstream effectors essential for vesicle formation, movement, tethering, and fusion.
• RAB14 is implicated in membrane trafficking between the Golgi complex and endosomes during early embryonic development.
• It regulates Golgi-to-endosome transport of FGFR-containing vesicles, a pathway critical for basement membrane deposition and epiblast/primitive endoderm lineage differentiation.
• Together with its guanine nucleotide exchange factor DENND6A, RAB14 controls the endocytic transport of ADAM10, N-cadherin/CDH2 shedding, and cell-cell adhesion.
  Related references: PMID:22595670
• RAB14 mediates endosomal tethering and fusion by interacting with RUFY1 and RAB4B.
  Related references: PMID:20534812
• An interaction with RAB11FIP1 suggests a possible role in neurite formation.
  Related references: PMID:26032412
• RAB14 localizes to recycling endosomes, early endosome membranes, the Golgi apparatus, trans-Golgi network, cytoplasmic vesicles, and phagosomes. It undergoes post-translational modifications including acetylation, methylation, and prenylation.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 39 – 140 of human RAB14) lies within a region that is conserved in mouse, which likely accounts for the observed cross-reactivity. When designing experiments, verify sequence alignment for any non-listed species.
• For Western blot, the predicted molecular weight is 24 kDa, but post-translational modifications or protein complexes can shift apparent migration. Include appropriate positive controls (e.g., human or mouse cell lysates) and consider using reducing/denaturing conditions.
• In ELISA, the polyclonal nature may provide high sensitivity. Optimize coating antigen and antibody dilution; using the antibody as a capture reagent paired with a different detection antibody may reduce background.
• Polyclonal antibodies can exhibit lot-to-lot variation. For critical or quantitative assays, it is advisable to test multiple batches and establish standard operating procedures.
• Ensure adequate sample preparation and blocking steps to minimize non-specific binding, especially when working with complex biological matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to a broad portfolio of IVD raw materials, including this anti-RAB14 rabbit polyclonal antibody. Beyond individual antibodies, we offer matched antibody pairs, bulk immunoreagents, and customized sourcing for rare or specialized targets, supporting your intramembrane trafficking and developmental biology workflows from discovery through validation.